Silvia Hoeboer Email & Phone Number

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Na een paar jaar als TOA te hebben gewerkt heb ik de switch gemaakt om de leerlingen niet alleen de praktische kant van de biologie bij te brengen naar de theoretische kant.

Bentinklaan 280, Rotterdam

Assisting in practicals, profile workpieces, maintenance of microscopes, inventory maintenance and other periphery and side issues concerns the practical part for the biology course.

Rotterdam, Nederland

Reactivation of fetal hemoglobin expression: functional analysis of candidate modifiers in vivo.Unraveling molecular aspects of gamma – beta globin switches during embryonic development and in adults in in vivo systems. Promotor Prof. J.N.J. Philipsen, co-promotor: T.B. van Dijk.Techniques used: DNA isolation, cloning, PCR, mRNA isolation, cDNA production, qPCR, Flow Cytometry, several mice handling techniques, protein isolation, pull downs, western blotting, cell culture, lentiviruses, transfection, CRISPR.

Leiden, The Netherlands

following several courses for graduation of my master degree

Brussels Area, Belgium

Laboratories of Dr. Cedric Blanpain under the supervision of Dr. G. Driessens.Title of project: Transcriptional analysis of human cutaneous squamous cell carcinomas.Techniques used: qPCR, immunohistochemistry, cloning, lentivirus production and infection.

Leiden, The Netherlands

At the dermatology laboratories and under the supervision of Dr.C. Tensen and Dr.F.R. de Gruijl.Title of project: Interspecies comparison of stem cell markers LGR5 and LGR6 in human and mice skin and hair follicles. Techniques used: qPCR, immunohistochemistry, mRNA in situ hybridizationStarted up the new KWF stem cell project of the dermatology department.

Rotterdam Area, Netherlands

Continuation of the internship Laboratories of Prof. dr. E.C. Zwarthoff under the supervision of Dr. H. Vékony. Title of project: Unraveling the mechanism of gene regulation by the leukemic oncogene MN1. Continuation of internship project. Techniques used: cloning, transient and stable transfections, luciferase assay, ChIP, qPCR, western blotting

Rotterdam Area, Netherlands

Laboratories of Prof. dr. E.C. Zwarthoff under the supervision of Dr. H. Vékony. Title of project: Unraveling the mechanism of gene regulation by the leukemic oncogene MN1, mainly on S100A8 and S100A9. Techniques used: cloning, transient and stable transfections, luciferase assay, ChIP, qPCR, western blotting

Rotterdam Area, Netherlands

Laboratories of Prof. Dr. J. Philipsen under the supervision of Dr. T.B. van Dijk. Title of project: Fop and its ‘friends’: and the role of the GAR domain. And production of GST-fusion proteinsProtein-Protein interaction between Prmt1 and FOP (Friend of Prmt1) by a GAR (Glycin Arginine Rich) domain of FOP. Making of GST-fusion proteins of Wdr18 en Senp3. Techniques used: cloning, transient and stable transfections, immunoprecipitation, western blotting, qPCR, immohisochemistryAcknowledged in “Friend of Prmt1, a Novel Chromatin Target of Protein Arginine Methyltransferases, van Dijk, T.B., et al, Mol. Cell. Biol. January 2010 vol. 30 no. 1 260-272

Doctor Of Philosophy - Phd, Biomedical Sciences, General

Verdediging van het proefschrift: Potential New Gamma-Globin Regulators: In vivo analysis of their role in the hematopoietic system.

Master, Life Science And Technology

Richting: Research and development

Bachelor, Biology And Medical Laboratoryresearch

Havo, Nature And Health, Havo

Atheneum, Nature And Health, Atheneum

Master Of Science, Leraar Vho In De Biologie