Team: Integrated Structural Biology Platform.o Responsible for the expression of proteins in insect cells (BEVS systems) for the benefit of the European infrastructure INSTRUCT.o Use and development of the BEVS system: generation of recombinant Baculoviruses (transfections, co-transfections) / amplification of a high titer viral stock / evaluation of recombinant Baculoviruses and optimization of expression at different scales and formats, including the production of multi-protein complexes.o Use and development transient expression in HEK 293 cells, FreeStyle 293 and Expi293 system for the production of secreted and intracellular proteins

o Carry out the R&D for the implementation of a functional prototype for the dosage of micropolluantso Rersponsable for the development, implementation and necessary verifications for the development of this prototypeo Scientific monitoring in the field of micropolluants

Team: Structural biology & genomics Platform.o Responsible for the expression of proteins in insect cells (BEVS systems) for the benefit of the European infrastructure INSTRUCT.o Use and development of the BEVS system: generation of recombinant Baculoviruses (transfections, co-transfections) / amplification of a high titer viral stock / evaluation of recombinant Baculoviruses and optimization of expression at different scales and formats, including the production of multi-protein complexes.

Team: Arnaud POTERSZMAN / Patrick SCHULTZ o Development of procedures for the production and purification of the endogenous protein factor.o Use of the CRISPR-Cas9 system: characterization and selection of clones / maintenance of cell lines: development of production and purification / biophysical characterization.

Team: Arnaud POTERSZMAN / Patrick SCHULTZo Development of procedures for the production and assembly of multi-protein complexes in order to study the functioning of the DNA repair pathway by nucleotide excision-resynthesis (NER).o Use of the Baculovirus system: production and purification of recombinant proteins / use of AKTA (FPLC).

o Many experiences in various structures as well as referent animator during several of these experiences.o Leading a team, organizing, framing and managing a group, knowledge of all audiences (from 3 to 17 years old) as well as disadvantaged backgrounds, sense of adaptation.

Team: Arnaud POTERSZMAN / Patrick SCHULTZo Production and purification of Replication Protein A (RPA).o Production and purification of proteins / electrophoresis on protein polyacrylamide gel / cloning by Gibson method, SLIC, RF-cloning, DNA maxi-preparation, bacterial transformation.

Team: Arnaud POTERSZMAN / Patrick SCHULTZo Manufacture of a DNA sequence carrying a mutation in order to study the NER functioning of the protein factor TFIIH.o Hybridization, elongation and ligation of oligonucleotides / electrophoresis on DNA polyacrylamide gel.