Simon Vial-Pradel Email & Phone Number

France

Saint-Malo

Continuation of the Doctorate subject. The projects can roughly be divided in two parts, the first one focuses on Protein-DNA in vitro interaction in order to identify targets of transcription factors and characterize the molecular interactions. The second one is based on the CRISPR/Cas9 technology. Special applications as transporter and creation of transgenic lines through controlled genome editing are both works in progress.

Kasugai, Aichi, Japon

Title of the thesis: Molecular and genetic study of ASYMMETRIC LEAVES2 and nucleolar factors in epigenetic regulation of Arabidopsis thaliana leaf development.Upper part (dorsal) and lower part (ventral) of leaf are two distinct tissues. The specialization of the undifferentiated cells and their proliferation along 3 axes result in the formation of flat and symmetric leaves. Fate determinant genes control this delicate process which require a precise balance between the factors involved… Show more Title of the thesis: Molecular and genetic study of ASYMMETRIC LEAVES2 and nucleolar factors in epigenetic regulation of Arabidopsis thaliana leaf development.Upper part (dorsal) and lower part (ventral) of leaf are two distinct tissues. The specialization of the undifferentiated cells and their proliferation along 3 axes result in the formation of flat and symmetric leaves. Fate determinant genes control this delicate process which require a precise balance between the factors involved. Repression of ventral fate determinant genes by dorsal fate determinant genes is very important for the establishment of the dorsal-ventral patterning. However, the molecular mechanism of this critical step in early leaf development is still unknown. Many factors involved in the repression have been identified and several of them genetically interact with an important dorsal determinant gene. Several approaches have been used for this study in order to unravel the underlying molecular mechanism, the knowledge gained from this study has important evolutionary implications for the land plants. And one of the most exciting prospects is the possibility of a dynamic repression mechanism that would occur at the early stage of leaf development and durably repress the target gene through epigenetic modifications. Show less

Tokyo, Japon

Promoting the French scientific influence in the Life Sciences field in Japan. Monitoring new technologies and research in order to keep French actors up-to-date through the writing of weekly reports. Writing a more comprehensive report on the state of Regenerative Medecine in Japan which is a domain that saw prominent Japanese Nobel prizes and promising clinical applications in development. Humbly representing France during conferences or MoU signatures and taking part of important… Show more Promoting the French scientific influence in the Life Sciences field in Japan. Monitoring new technologies and research in order to keep French actors up-to-date through the writing of weekly reports. Writing a more comprehensive report on the state of Regenerative Medecine in Japan which is a domain that saw prominent Japanese Nobel prizes and promising clinical applications in development. Humbly representing France during conferences or MoU signatures and taking part of important representatives visits to Japan. Show less

Kasugai, Aichi, Japon

Study of the molecular mechanisms of leaf development of Arabidopsis Thaliana. Dynamic analysis of the epigenetic modifications of a gene in relation with plant lines and environmental parameters thanks to molecular biology tools. Work on DNA and RNA, use of plant and bacterial cultures and RNA chips and PCR. Languages used: English and Japanese. Frequent presentation of the results in English. The data I produced brought better understanding of particular mechanisms and helped to orientate the… Show more Study of the molecular mechanisms of leaf development of Arabidopsis Thaliana. Dynamic analysis of the epigenetic modifications of a gene in relation with plant lines and environmental parameters thanks to molecular biology tools. Work on DNA and RNA, use of plant and bacterial cultures and RNA chips and PCR. Languages used: English and Japanese. Frequent presentation of the results in English. The data I produced brought better understanding of particular mechanisms and helped to orientate the next steps of the project and contributed to a publication. Show less

Munich Area, Germany

Small company experience. Execution of bioassays for pharmaceutical candidates characterization. Produced data has been used to feedback the database and optimize the design of new chemicals. Work in autonomy with regular report to the hierarchy. Languages used: English and German. Programming and use of automated devices to perform assays and analyses according to the protocols. Various projects including ELISA, Lantha, Adapta, Omnia and cellular based assays.

Research Doctorate, Molecular Biology

Master'S Degree, Chemistry