Simone Kirkland Email & Phone Number

Basel, CH

• Develop and execute experiments including, but not limited to, assessment of enzymatic assays using a variety of detection techniques, separation and detection technologies for biomolecules, and equipment assessments
• Develop and perform data analysis and contribute to presentations including summaries with conclusions and recommendations
• Establish and maintain good working relationships and open communication with other departments such as Marketing, QC, QA, and Operations
• Support other R&D projects within my expertise and experience. Develop and improve new skills and knowledge necessary to successfully support assigned projects

Gaithersburg, Maryland, US

• Performed analytical testing using SDS-PAGE, Western Blot, fluorescent, visible and UV basedspectrophotometric methods, impurity assays (such as host cell protein ELISA and host cell DNA quantitation), and protein.
• Maintained cell culture lines obtained from human embryonic kidney cells (HEK), adenocarcinomic alveolar basal epithelial cells (A549), African green monkey kidney epithelial cells (Vero), and Sf9 cells (derived from.
• Executed plaque assay and infectious unit assays following established departmental Standard Operating Procedures and Test Methods
• Assisted with lab organization, maintenance, and participate in 6S activities
• Executed experiments for technology transfer and method validation of host cell protein assays
• Summarized and analyzed test results utilizing a sample submission database to report test results

• Performed ELISA on clinical samples to quantify relative levels of Protein Kinase C ε (PKCε) in order to distinguish Alzheimer’s Disease (AD) from Non-AD dementia (Non-ADD) and Age-matched control (AC) specimens
• Established adherent primary cell culture obtained from human skin fibroblasts and maintain over 50 cell lines in preparation for total protein quantitation assay and ELISA
• Organized and maintained records of patient information and laboratory equipment use in compliance with Clinical Laboratory Improvement Amendments (CLIA), Health Insurance Portability and Accountability Act(HIPAA), and.
• Participated in a blind FDA trial in order to validate the results of 37 cell lines that had previously been diagnosed as AD or AC

Washington, DC, US

• Maintained IPEC-J2 cell tissue culture and tracked morphology and confluence over time to use as a model for human enteric epithelium
• Isolated single colonies of fungal populations in the feces of swine from birth through the critical weaning transition to investigate if certain fungal populations were associated with poor weaning outcome
• Extracted DNA from tail, small intestinal, and liver tissue of transgenic mice to subsequently perform PCR genotyping
• Created and tested the enzymatic activity of Clostridium perfringens-specific endopeptidase chimeras to target bovine mastitis-causing bacteria
• Routinely conducted laboratory maintenance practices, including autoclaving and properly disposing of biohazardous waste and taking chemical inventory

• Extracted DNA from transgenic Drosophila lines to use as models for human kidney diseases, congenital heart diseases, and leukemia
• Experimentally studied fly nephrocyte function and development through observing the effects of feeding Drosophila a combination diet of high sugar and silver nitrate
• Performed antibody staining to detect proteins encoded by candidate genes for heart disease
• Experimentally studied the phenotypes of risk alleles for APOL1-associated renal diseases
• Cloned candidate genes for kidney disease in order to transform a Drosophila cell with the recombinant plasmids

• Conducted Quality Assurance for case files from the AIRP database and charged with PHI digital redaction of medical forms to comply with HIPAA (Health Insurance Portability and Accountability Act of 1996) standards
• Edited PowerPoint documents for staff radiologists in preparation for course lectures
• Extracted and transported physical case files for use by resident pathologists as well as executive staff