Sofia Dente Email & Phone Number

Nottingham, England, United Kingdom

• Research and development of Recombinant Albumin formulations, and Viral Vectors (including AAV)• Analysis of particulate contamination and stability of Recombinant Albumin formulations, and Viral Vectors • Use of Microscopy, PCR, ELISA, LAL, Chromatography, Ultrafiltration, Diafiltration, Fluorescence, Spectrometry• Use of Halo Aura GT, Wyatt DLS, Wyatt SEC-MALS, Agilent HPLC, Thermofisher Dionex HPLC, Cytiva AKTA, Brightwell Micro-Flow Imaging™(MFI), Thermofisher Applied Biosystems 7500 Real Time PCR, BMG Fluostar OMEGA Plate Reader, Charles River Endosafe nexgen-MCS, Sartorius TFF, Varian Cary 50 UV-VIS Spectrophotometer• Management of COSHH, Risk Assessments, and Biological Risk Assessments • Design of Experiments, writing of reports, presentations, and laboratory notebooks, data analysis, understanding and writing of patent documents• Coaching, training and support of colleagues • Laboratory management including sourcing consumables, ordering, and organising maintenance and calibration of instruments, cleaning and sanitisation of biological entities• Working at Biological Safety Level Two, working to GMP standards • Management of own workload; lone working• Mental Health First Aider• Fire Marshal • Gas Cylinder Handling • Member of Health and Safety Committee

Nottingham, England, United Kingdom

• Receipt and data entry of nasopharyngeal swab SARS-CoV-2 samples into LIMS• Transfer of neat sample media to 96-well reaction plates• Nucleic acid extraction and purification using Thermofisher TaqPath™ assays, ThermoFisher KingFisher™ Flex and QuantStudio™ 5• Pipette use and maintenance including various volumes, multi-channel and electronic pipettes• Class II Biological Safety Cabinets use and maintenance • Completion of environmental checks, reagent formulation, waste management and cleaning records• Working in a Biosafety Level Two laboratory• Working to a UKAS ISO/IEC 15189 accredited standard

Worksop, England, United Kingdom

• Ensuring micro sampling plans are maintained by collecting and testing of samples and mitigating adverse results through investigation• Monitoring product quality, identifying trends and generating actions plans with relevant departments to address issues• Completing audits to schedule - internal audit/Customer Codes of Practice, HACCP/CCP, GMP, FB, Hygiene Fabrication, Glass, and Plastic• Controlling traceability through documentation, mass balance and equipment provenance, and packaging • Coordinating taste panels for products and ensuring that product launches are prepared for and have a smooth transition from process to production• Managing food safety through routine temperature checking, weight calibration, and escalating factory and quality incidents

Sheffield, England, United Kingdom

• Decanting neat urine and transferring swab samples into stabilising PCR solution • Centrifuging blood samples and decanting serum into false bottom tubes • Sorting, booking and archiving of samples • Logging and reporting problem samples and non-conformance incidents to the Quality Officer • Investigation of non-conformance incidents • Supporting the Quality Officer with non-conformance write up• Adhering to the requirements of confidentiality disclosure agreements using GDPR • Correct manual handling and disposal of confidential and hazardous waste • Performing and recording stock checks • Supporting Senior Laboratory Technicians operating Roche technologies as directed • Working to a UKAS ISO/IEC 15189 and ISO/IEC 17025 accredited standard • Answering Service User queries via email, obtaining security information and navigating private record systems

Nottinghamshire, England, United Kingdom

• Preparation of plane blades, vanes and other samples using; Struers Discotom Cutters, Citopress, Grinders and Polishers, Vacuum Impregnation System, and cold and hot mount resin • Analysis of coatings under Light Microscopy; taking of thickness measurements, and analysis of grit contamination, metallic contamination, coating structure and columnar structure • Analysis of coatings under Scanning Electron Microscopy using Point ID, and Elemental Mapping• Cyclic testing of parts at 1190°C to assess coating bonding and stability• Conversant with INCA, AZTEC, Microsoft Access, IMS and Shop Tracker software• Use of SOPs, CMEs, Route Cards, and Data Cards• Stamp authorized to assess work, over check work, and move batches to the next stage of production• General laboratory housekeeping including stock procurement, and equipment calibration and maintenance

Burton Upon Trent, Staffordshire, United Kingdom

• Extraction and preparation of water and soil samples using UKAS accredited methods for the assessment of sample volatility levels using GCMS and HPLC • Handling of Radioactive Substances, Japanese Knotweed (Fallopia japonica), and Asbestos• Sample acceptance and organisation• Data input, measuring and note taking• General laboratory housekeeping including stock procurement, vialing and stock rotation, and equipment calibration and maintenance• Solo working to a UKAS ISO/IEC 17025 accredited standard

Archer Road, Sheffield, South Yorkshire, United Kingdom

Customer service position using the same skills as described previously.

Sheffield, South Yorkshire, United Kingdom

• Till use, sale of Restricted Items, and Banking• Money handling, tender authentication, and sales of restricted items• Stock rotation, shelving, dressing, and cleaning duties • Customer service both in store and over the phone• Safe and Legal training including DEFRA, chill/freezer best practice, emergency procedures, fire safety, and the laws related to the sale of restricted items • Equipment usage and maintenance• Ordering and receiving of deliveries from depot, and external sources• Coaching, Team Management, and Key Holder • BIIAB Level 2 Award for Personal License Holders• TQUK Level 2 Award in Emergency First Aid at Work (QCF) (Expiry – 7/10/2019)

Sheffield, South Yorkshire, United Kingdom

• Bat surveying including transect, static monitoring, nocturnal and building surveys• Great Crested Newt surveying including bottle trapping, torching and HSI calculations• Reptile, nesting bird and wildflower surveying • Survey skills used in various terrains including buildings, grasslands and river systems via foot and boat • Report annotation and letter report formation• Data consultation requests and data compilation• Transect map, survey position and photo sheet figure design• DAFOR, BOCC and Schedule 9 table creation• Sonogram analysis, bat activity calculations, fecal identification and DNA petition• Infra-red camera set up and video analysis for bat activity• Use of Analook, BatExplorer, SASLab, BatSound4 and ARCGiS softwares• Conversant with multiple acoustic recording models including Anabat, EM3+, BatLogger, EchoTouch and R05 systems for nocturnal, transect and static surveys

Sheffield, South Yorkshire, United Kingdom

• Till use, sale of Restricted Items, and Banking• Money handling, tender authentication, and sales of restricted items• Stock rotation, shelving, dressing, and cleaning duties • Customer service both in store and over the phone• Safe and Legal training including DEFRA, chill/freezer best practice, emergency procedures, fire safety, and the laws related to the sale of restricted items • Equipment usage and maintenance• In-store baker

Dronfield, North East Derbyshire, United Kingdom

• Money handling, tender authentication, sale of restricted items• Customer service, resolving customer complaints• Food handling and preparation• Cleaning of machinery, stock rotation

Masters (Msc), Biotechnology, Merit

Virological based Thesis; The mutagenesis of Escherichia coli strain F16 as a way to create a model organism; which may be used.

Bsc (Hons), Zoology, 2:1

Activities and Societies: Student ambassador (2011 - 2014). Treasurer of the Ecology Society (2012-2013) Vice President of the Ecology.

Education record

Activities and Societies: Library assistant, Council member, Volunteer school bank member for NatWest