o Immunoassays like Neutralization of HIV in TZM-bl cells, ELISA and ELISpot o Data entry, cleaning, analysis, interpretation and presentationo PBMC isolation and plasma preparation from whole bloodo Flowcytometry and Intracellular Cytokine Staining (ICS)o Pseudovirus production by co-transfection in 293T cellso Writing and review of study associated documentso Sample reception, processing, storage and shipmento DNA extraction, Molecular cloning and sequencingo Operation and maintenance of complex equipmento Expansion of plasmids using E. colio Manuscript writeup and review

o Immunoassays like Neutralization of HIV in TZM-bl cells, ELISA and ELISpoto Cleaning and disinfecting laboratory surfaces and equipmento Pseudovirus production by co-transfection in 293T cellso Scientific data entry, cleaning and managemento Liquid Nitrogen and temperature monitoringo Expansion of plasmids using E. colio PBMC isolation from plasmao Stock taking of reagentso Specimen shipment

o Flowcytometryo Proposal and dissertation writingo Stimulating, staining and preparing samples for acquiringo Data generation, analysis, interpretation and presentationo Computer applications like SPICE, FlowJo, GraphPad and Pestle