Gene therapy development for deafness - Designed, constructed and recombined plasmids encoding therapeutic candidate- Transfected cells and measured the reporter gene activity by luminescence to compare the efficacy of three specific promoter in vitro +/- one enhancer +/- four intronic sequences- Performed in vivo injection of adeno-associated virus (AAV) for gene therapy optimization and/or proof of concept : o tropism study with 5 capsids o AAV encoding therapeutic sequence - Conducted mouse phenotyping experiments to access hearing function (ABR, DPOAE)- Isolated and labelled mouse cochlea to observe their integrity and localised important protein or RNA- Optimized route and age of administration for surgical procedures - Contributed in transgenic mouse genotyping- Initiated western-blot from cell pelletLeadership : correspondent hygiene and safety for my team.

Research associate in a lab producing advanced therapy medicinal products for blood disease (Cell Therapy and Gene Therapy lab (LTCG), Pr. Cavazzana)- Operating control analysis of advanced therapy medicinal products for cell and gene therapy (sterility, cell numeration, viability, immunolabelling, ability of cells to proliferate)- Performing patients follows up : separation and quantification of cells, bio banking, cell characterization (according to therapy : determination of vector copy number, functional testing, ability of cells to proliferate) - Classifying the data set relative to the lab activity (before and after therapy and during the production of advanced therapy medicinal product)- Conducting validation of procedures in accordance with GMP with other team members : risk assessment, experimental plan- Supporting quality management system : procedures proofreading and upgrading

Characterization of the receptor MERTK involved in retinal phagocytosis. - Engineered 40 plasmids encoding for MERTK receptor carrying specific mutations- Isolated cells from rat and pig eyes and maintained mammalian cell lines - Transfected cells and characterised the effect of MERTK receptor mutation on its expression and activity- Performed genotyping experiments of 4 rodents’ lines and tested different genotyping procedures with the animal facility to reduce animal pain- Conducted rodent phenotyping experiments to observe visual function (SLO, OCT, micron) - Optimized protocols used for transfection and immunolabelling - Contributed in the miniaturisation of phagocytosis test on plate for high throughput screening- Initiated the development of a new phagocytic flow cytometry-based test in collaboration with core facility- Supervised undergraduate studentsLeadership : comanager of hazard room (EtBr)

Understanding Polycomb complex redeployment in Drosophila , production of recombinant Su(z)12 andDSP1 proteins to be used for antibody production.- Designed constructed and recombined plasmids for protein production - Produced in bacteria and purified Polycomb complex subunits SUZ12 and DSP1- Isolated Drosophila imaginal discs and prepared samples for ChIP-qPCR- Adapted and optimized production conditions according to protein properties and specificities - Supported Drosophila maintenance and crossingLeadership : lab manager: organized and maintained common stocks, ordered supplies and equipment, helped with missions’ organization.

Gene expression inhibition with shRNA in a large panel of human cell lines.- Implemented lentiviral transduction, now routinely used in the laboratory- Generated stable knocked-down cell lines using shRNA targeting a receptor expression - Quantified gene expression inhibition at the mRNA and protein level - Improved inhibition with variation of MOI and cloning of cells

Assessment of the impact of different hypothermic preservation on hepatocytes for liver cell therapy- Conducted cells cryopreservation in 3 different media with different freezing and defrosting protocols - Performed cells hypothermic storage in 3 different media testing different cell concentrations and times of preservation - Monitored hepatocyte viability and assessed their adherence and protein secretion ability.- Assessed human hepatocytes migration capacity and their ability to implant and secrete proteins in vivo in a mouse model