Somes Kumar Das Email & Phone Number

San Diego, California, United States

Long DNA assembly in CMOS chip

San Diego Metropolitan Area

Worked on multiple research projects (i) Isothermal amplifications, qPCR, (ii) CRISPR-Cas system-based assays for diagnostics (iii) Glass surface modifications. Immobilization of biomolecules on glass surfaces: (iv) platform for spatial multiplexing: Proof of concept (v) Nanopore based multiplexed detection of amplified target sequences: proof of principle.

South San Franisco

Worked on a project of lipid bilayer formation and stabilization on nanopore arrays, surface modifications (functionalization, coating, molecular layers) and characterizations (XPS, Ellipsometry/ surface energy/contact angle, EIS, CV) of nanopore devices. Additionally, helped in the development of sequencing technology by single molecule detection using.

Greater St. Louis Area

Led multiple R&D projects (finished/continuing): (i) Biochemical assays, biophysical analysis methods for NAs; multiplexing assay of tens of NAs by nanopore based single molecule counting; (ii) key role in instrument and nanopore arrays device development and their integration in the point-of-care diagnostics tool (iii) Trained junior scientists (iv).

Lexington, Kentucky Area

Led multiple research projects including (i) photohysical characterizations of carbon nanodots, and (ii) conformational dynamics and mechanism of interactions of ion channel membrane proteins in live cells by single molecule fluorescence imaging and ion channel electrophysiology on nano-apertures based zero-mode-wave-guides (ZMW) and in in vitro lipid.

• Strongly involved with the R&D team in method and assay development for the single molecule DNA structural analysis and mapping by fluorescence imaging in nano-fluidic channel arrays.
• Took leading role in designing and developing new, highly efficient fluorescent labeling schemes compatible to the Company’s NanoAnalyzer and nanochannel chip for high content fluorescence imaging to extract the.
• Acted as the key person in the advanced technology group to… Show more
• Acted as the key person in the advanced technology group to explore and develop new technology, applications, assay and protocols by providing intellectual and creative solutions, and research support. Designed.
• Established and optimized new method and protocols starting from the sample preparation to nano-channel loading (achieved maximum resolvable occupancy from 3% to ~10%) and fluorescence imaging of long DNA molecules for.
• Improved DNA polymer stretching efficiencies in nano-channel, assessed alternative high throughput DNA stretching strategies, and developed understanding of the fundamental physical principles relevant to DNA.

Worked as laboratory head to setup a research laboratory to study membrane proteins utilizing single molecule fluorescence and single channel electrophysiology techniques. Built setups for TIRF, FRET and patch clamp experiments. Designed and executed experiments and analyzed data. Drafted scientific proposals, managed research projects, and prepared.

Research on the thermodynamics of membrane protein (bacterial toxins) binding to lipid membranes at different pH in presence of fluorinated surfactants using various spectroscopy tools including single molecule fluorescence correlation spectroscopy (FCS, autocorrelation and cross correlation), fluorescence resonance energy transfer (FRET) and fluorescence.

Established single molecule imaging laboratory. Prepared a lipid bilayer setup to study oligomerization of pore forming toxins on lipid membrane using single molecule fluorescence microscopy (single molecule photobleaching, single particle tracking) and single channel electrophysiology. Designed and optimized experimental protocols, did experiments.

Ph.D, Biophysical Chemistry

Master Of Science (Ms), Physical Chemistry