Somya Singh Email & Phone Number

Menlo Park, Ca, Us

Menlo Park, California, Us

Menlo Park, California, Us

Ithaca, Ny, Us

* Investigated the effect of low temperature on casein kinase mutants.* Quantification of biomass and rosette size of CDPKs.* Analyzed cis-element of SNC1 .* Effective presentation of research work at team meetings.

• Ability to work independently with an ability to design and optimize experiments for comparative epigenomics between drought-susceptible (IR64) and drought-tolerant (N22) rice varieties. • Optimized protocols for sample preparation and library preparation from rice tissues for next generation sequencing (NGS). Made next generation sequencing libraries for DNase-seq, MNase-seq and ATAC-seq.• Designed, lead and managed experiments for genome wide profiling of histone modification marks in Oryza sativa during drought stress.• Troubleshooting expertise with in-depth knowledge and advanced experience in molecular biology, genomics and proteomics resulting in the successful completion of wet lab experiments of 2 research projects.

Delhi, Delhi, In

Projects Undertaken:• “Transgenic approaches for Frost Resistance in Indian Mustard.” Evaluated transgenic Brassica juncea plants for their capacity to withstand frost stress. Cloned known positive and negative regulators of cold stress pathway under the control of either embryo-specific Napin promoter or stress-inducible RD29A promoter to introduce frost tolerance in B. juncea. Standardized conditions of radiative frosting, in a frost chamber, to mimic frosting in fields and also optimized stress regimes to induce lethality in untransformed plants. Tested untransformed and transformed B. juncea lines for frost tolerance, under standardized stress regimes, which resulted in few plants showing high frost tolerance as compared to the wild type • “Transcriptome profiling of Brassica juncea siliques during cold stress andvarious developmental stages.”• “Isolation of cold-inducible embryo-specific promoter.” Used genome walking approach to identify and clone a cold-inducible and embryo-specific promoter from B. juncea. • “Unravel the dynamics of chromatin architecture in response to heat, salt, drought and cold stresses in Oryza sativa by using two cross validating methodologies [DNaseI hypersensitive assay (DNase) and Formaldehyde Assisted Isolation of Regulatory Elements (FAIRE) coupled with high throughput sequencing]”Further superimposed the transcriptome information on the identified open chromatin regions (OCRs), to decipher importance of various cis-elements in regulating gene expression.• “Mutant analysis and open chromatin study during abiotic stresses in Arabidopsis.”

Master Of Science - Ms

Doctor Of Philosophy (Phd)