Sonal Tonapi Email & Phone Number

Leading the development of new workflows for affinity reagent development and characterizationLeading cross function teams to drive organization critical projects forwardManaging a cross functional team of scientistsDefine and prioritize team goals, timelines to strategically drive projects forwards

San Francisco Bay Area

Led improvements and expansion of affinity reagent development pipelinesMentored a large team of early career scientists Planned and executed multiple strategic projects with CROs

San Francisco Bay Area

Established novel high throughput workflows for affinity reagent selection

Phoenix, Arizona

Phoenix, Arizona

• Non-invasive cancer profiling using the ADAPT Biotargeting System™ • Drug target identification in vitro using single aptamers in Non-Hodgkin lymphoma• Examined aptamer therapeutic potential and mechanism of action in Non-Hodgkin lymphoma• Developed high-throughput enzyme based assay to validate aptamer protein targets • Data analysis • Presented study data at multiple national and international conferences• Techniques used include: Viability assays, Aptamer based pulldowns of protein targets, RNAseq, ELONA, western blot, SELEX, mass spectrometry, qRT-PCR

San Antonio, Texas Area

Identified 53BP1 function in maintaining normal progression of replication by stabilizing and protecting stalled forks and the impact of these processes on spontaneous homology direct repair. • Established primary cultures from mice• Studies DNA damage response and repair in vitro and in vivo • Examined replication progression in vitro• Data analysis: Microsoft Office, Prism • Techniques used include: tissue culture, viability assays, immunofluorescence microscopy, PCR, ChIP-PCR, transfections (plasmid and siRNA), western blotting, DNA fiber analysisExamined the consequence of chronic endogenous stress due to DNA damage response and DNA repair deficiencies on increasing incidence of cancer and metabolic diseases using the Ataxia Telangiectasia disease model. • Examined diabetes progression in vivo using the Ataxia Telangiectasia disease model• Identified the mechanism of diabetes onset in vivo and in vitro• Examined the effect of small molecules on disease incidence and progression• Managed a large mouse colony of various constitutive and conditional knock-out models• Techniques used include: mouse glucose and insulin tolerance tests, western blot, ELISA, RNAseq, immuno-purificationMentored Undergraduate summer internsTeaching assistant: Cancer Biology Core I

Washington D.C. Metro Area

Investigated the differential expression of ID3 splice variants on squamous cell carcinoma progression• Examined the expression of ID3 isoforms in normal keratinocytes and squamous cell carcinoma lines • Studied the role of ID3 isoforms on proliferation and apoptosis in vitro• Techniques used include: Tissue culture, western blot, RT-PCR and plasmid propagation.Tutored undergraduates in General Chemistry

Doctor Of Philosophy (Phd), Oncology And Cancer Biology

Master Of Science (M.S.), Biochemistry And Molecular Biology

Bachelor Of Science (B.Sc.), Chemistry