Sonja Mihailovic, Phd Email & Phone Number

California, United States

• Designed and led a multi-disciplinary team project to create novel methods to improve pluripotent stem cell tissue and organoid differentiation efficiency into hematopoietic, neural, endothelial, immune, and cardiac.
• Independently developed a scalable alternative for iPSC cardiac differentiation into micro-cardiac organoids that increased target drug screening 20-fold and reduced reagent use by 90%.
• Established pipeline for CRISPR/Cas9 gene editing in mammalian cell cultures and iPSC lines involving knock-downs,-outs, and knock-in live fluorescent reporter tags to increase integration efficiency, survival, and.
• Individually organized and presented internationally-promoted large-scale webinars, performed customer technology demonstrations, and established scientific collaborations with multi-disciplinary field experts.
• Generated personalized disease models for neurodegenerative diseases using patient-derived neural organoids coupled with cell-based assay quantification and CRISPR/Cas9 gene editing for potential corrective therapy.

• Created and executed research project involving embryonic organoid development, reprogramming, and multi-lineage iPSC differentiation focused on gastruoids / gastrulation models utilizing constructed.
• Led cross-functional group to process large-scale data analysis and visualization of real-time iPSC gastrulation and trilineage differentiation with Python on tracked single-cell and organoid confocal microscopy.
• Designed drug screening to generate iPSCs from patient-derived cells which resulted in successful hit compound that was utilized to launch Nucleate start-up venture program.
• Produced lentivirus-derived CRISPR/Cas9 knockout lines to identify epigenetic SNPs related to ANCA vasculitis patient remission that resulted in 80% remission prediction accuracy.

Chapel Hill, North Carolina, United States

Managed and directed senior undergraduate students in a cancer genetics course project involving current literature review on novel oncogenes and organized potential experimental designs in-person and remotely.

Research Triangle Park, NC

• Led a business-oriented team to pursue a potential biotech start-up from current experimental discoveries with novel alternative small molecule reprogramming product for hiPSC generation.
• Implemented translatable business skills associated with bio entrepreneurship including polished pitching, understanding regulatory strategies, and assessing technical and market risks with the Nucleate Activator.

Tallahassee, Florida, United States

• Developed novel cellular assays for tumor detection in Drosophila cancer models to increase detection accuracy by 35%.
• Evaluated neuronal developmental effects of X inactivation by generating knockdown lines using CRISPR/Cas9-gene editing.
• Taught, trained, and mentored students in developmental genetics, microscopy, and experimental design.

Doctor Of Philosophy - Phd, Genetics And Molecular Biology

Bachelor Of Science - Bs, Biology/Biological Sciences, General