Sophia Moore Email and Phone Number

Focusing on soft tissue surgery, dentistry and emergency medicine. Helping provide an urgent care for Lacey and Olympia. Providing CPR training for Lacey and Olympia clinics as well as for Field Leaders.

High volume soft tissue surgery days once per month to keep up routine surgical skills and preventative care medicine.

-Preventative care: vaccinations, heartworm/flea prevention, deworming, educating clients, health certificates, wisdom panels, routine bloodwork and radiographs, helped organize and run vaccine clinic-Surgical experience: small and large dog/cat spays and neuters, pyometra, gastropexies, cherry eye procedures, stenotic nares wedge resections, mass removals, punch biopsies, gingivectomies, dental extractions, umbilical hernia repairs, laceration repair, abdominal and inguinal cryptorchid neuter, limb amputation, cystotomy, gastrotomy, enterotomy, enucleation, etc.-Medical experience: diagnosing and/or managing diabetics, Cushing's, hyperthyroid cats, CKD in cats and dogs, hepatic diseases, ophthalmic (bacterial conjunctivitis, corneal ulcers, foreign bodies etc.), abscesses, anal gland abscess, bandaging and wound management, wet tail in hamsters, upper respiratory disease and ringworm in guinea pigs, humane euthanasia, dermatology cases (demodetic mange, flea infestation, atopic dermatitis and food allergies, fungal infections, mastitis, pyoderma etc.), oncology workup and management (lymphoma, mammary carcinoma, mast cell tumor, thyroid carcinoma, etc,), behavior modification, neuro/orthopedic (vestibular syndrome, IVDD, arthritis, angular limb deformities, patellar luxation, cruciate injuries, seizure patients), obesity, gastrointestinal disease (giardia, coccidiosis, HGE, IBD, pancreatitis etc.), urinary issues (UTI, urinary calculi, spay incontinence, behavioral marking), dental disease (retained deciduous, fractured teeth, oronasal fistulas, periodontal disease etc.) and more!-Leadership: working with LVTs, managers and fellow associate veterinarians to increase efficiency and resolve interpersonal conflicts in the hospital, participated in CE Confronting Conflict-Conferences: Dove Lewis Conference 2018, 2019 Blue Pearl Symposium Seattle, International Veterinary Seminar Seattle 2019-Proficient with PetWare

-Performing emergency surgeries including pyometra, foreign body, C-section, splenectomy, tarsorrhaphy, enucleation, laceration repair, urethral prolapse, vaginal prolapse, GDV, rectal prolapse purse string. -Diagnosing and treating/managing salmon poisoning, vestibular syndrome, CHF, kidney failure, HBC, dystocia, toxicities (chocolate, Adderall, marijuana, lily, grape, rodenticide, xylitol, meth, fentanyl, organophosphate, etc.), aspiration pneumonia, heat stroke, rectal prolapse, big dog little dog trauma, blocked cats and dogs, saddle thrombus in cats, cranial cruciate injuries, fractures and other orthopedic trauma, hepatic lipidosis, constipation, DKA, UTI, FLUTD, aural hematoma, anaphylaxis, parvovirus, feline panleukopenia, FIP, anal gland abscess, other abscess, IVDD, HGE, IMHA, corneal ulcer, diabetes, Addison's etc.-Collaborating with board certified specialists from all departments including surgery, oncology, internal medicine, and cardiology. -Training new doctors and ensuring quality medicine -Performing care simultaneously for outpatient triages and critical care inpatients-Proficient with EzyVet and Smart Flow-Current certification for RECOVER CPR ALS and BLS by the American College of Veterinary Emergency and Critical Care-Making the DVM schedule and coordinating with relief doctors-Created a template on Google Drive Excel to streamline transfers between Specialty Departments, assist in more organized rounds, and help efficiency in writing SOAPs for ICU. And created a training video for how to use it.

I worked with Dr. Christopher Allen on research projects involving delayed homologous recombination in RKO colon carcinoma cells after exposure to low linear energy transfer (LET) X-ray and and high LET carbon and iron ion ionizing radiation (IR). Wet lab and analysis experience:-Preparation of genomic DNA from cell lysates-Cleanup of PCR products for sequencing using purification columns-PCR and gel electrophoresis-Using Image J, Adobe Photoshop and illustrator to generate publication ready figures of time-lapse images-Utilizing the Proteomics and Metabolomics Facility for DNA sequencing of carbon and X-ray PCR products -Sequence Analysis using Snap Gene and Blast-Posters:High LET ionizing radiation generates higher levels of delayed homologous recombination in mammalian cells. Heavy Ion in Therapy and Space Radiation Symposium 2013. Allen C, Hirakawa H, Nakajima N, King M, Moore S., Sugiura M, Hoki Y, Araki R, Abe M, Nickoloff, JA and Fujimori A. Chuo-ku, Chiba-shi, Japan, 5/15-5/18/2013.Low and High LET Ionizing Radiation Induces Delayed Homologous Recombination That Persists for Two Weeks. Allen C, Hirakawa H, Nakajima N, King M, Moore S., Sugiura M, Hoki Y, Araki R, Abe M, Nickoloff, JA and Fujimori A. 2013 International Open Laboratory Progress Report Meeting, Inage-ku, Chiba-shi, Japan, 1/22/2013.-Grant submissions:2013 CSU Cancer SuperCluster, Flint Animal Cancer Center and CSU Ventures, “Interrogating the exome and methylome for alterations that drive radiation-induced delayed genomic instability,” submitted May 10, 2013.College of Veterinary Medicine and Biomedical Science: College of Research Council 2013 College Research Proposals, “Interrogating the exome and methylome for alterations that drive radiation-induced delayed genomic instability,” submitted March 25, 2013.

This summer I am collaborating with both the Roslin Research Institute in Dr. Argyle's laboratory and here at the Prion Research Institute at Colorado State University in Dr. Mark Zabel's laboratory.

I was awarded the RDSVS summer research fellowship exchange in Edinburgh, Scotland funded by College of Veterinary Medicine and Biological Science at Colorado State University for the summer of 2014. I am working under Dr. David Argyle in collaboration with Dr. Wilfred Goldmann's lab to investigate the potential role of cellular prion protein (PrPc) in canine cancer.Wet lab and analytical experience:- Western blot analysis using luminescence and Li-cor (Sandra Wishart)- Indirect Flow Cytometer (BD LSRFortessa x-20) trained by Anna Raper at Roslin- FlowJo trained by Anna Raper at Roslin-siRNA trained by Heather Bender at CSU -Homogenization of canine brain trained by Dot K at Roslin-PNGase -Harvesting and lysing cells for Western blot analysis uinsg NP-40 lysis buffer-Doxorubicin and ionizing radiation experiments at Roslin -Cyclohexamide experiments at RoslinFunding for project and presentations:1. CVMBS Summer Exchange program scholarshp with RDSVS (CSU)2. ARCS Foundation CVMBS Scholarship (CSU) 3. Presenter at 2015 Prion Conference (http://prion2015.org) 4. Presenter for summer scholars research day presentations - awarded 1st prize - July 27, 2015 in Fort Collins, CO 5. CVMA Student research poster competition - awarded 3rd prize, Sept 19, 2015 in Loveland, CO 6. Presenter at the Merial-NIH Conference - July - Aug 2, 2015 - UC Davis, California

I worked as reservationist at the Rocky Mountain Holiday Tours in Fort Collins, helping international clients mostly from Europe book and confirm hotels and activities in Western United States.

-Trained new canvassers -Navigated for the driver to the turfs-Ensured registered voters had received their ballot and were informed on the issues such as Amendment 65 both in English and Spanish

Investigating protein stability and degradation of Type A-ARRs (Arabidopsis Response Regulators) in the context of plant immunity (supervised by Dr. Cris Argueso)._______________________________________________________-Performed PCR, gel electrophoresis, protein extraction, cTAB DNA extraction, Western Blot, Inoculation with Hyaloperonospora arabidopsis, media preparation, sterilization of seeds, DNA digestion with restriction enzymes, plating of bacteria Gateway Vectors for single colonies, bacterial transformation using DH5a cells, and genotyping using PCR-based molecular markers -Led and conducted experiments-Presented project in Lab Meeting

Stem Cell Cryopreservation Protocol Revisited: A comparison of current cryopreservation of stem cells to a proposed technique incorporating the addition of cholesterol and propylene glycolTested the effects of the cryoprotectant propylene glycol (PG), membrane protecting cholesterol-loaded β-cyclodextrin (CLCs), and trehalose on the cryosurvival of stem cells. Previous studies reported PG was superior to DMSO for cryopreserving mouse stem cells. Therefore, I hypothesized PG would be more effective than DMSO when freezing three different stem cell lines. Other studies testing the effects of CLCs on Chinese Hamster Ovary cells and bull sperm led to my hypothesis that increasing membrane cholesterol content would result in higher cryosurvival rate. The stem cells tested were uncryopreserved horse mesenchymal stem cells and previously cryopreserved human and mouse trophoblast stem cells. The control treatment was the standard 10% DMSO while the experimental groups were frozen in 10% DMSO + CLC, 7.35% PG, and 7.35% PG + CLC. Cells were cooled to -41°C at -1°C per minute and stored in liquid nitrogen for 1-3 weeks. Cryosurvival rates were determined using flow cytometry. DMSO-treated cells survived freezing more effectively than PG for all three stem cell lines. Interestingly, the previously cryopreserved BeWo human trophoblast stem cells showed an increase in cell viability with the addition of CLCs with both DMSO and PG. Although the results of the horse and mouse stem cell experiments indicate that CLC and PG do not increase stem cell viability after cryopreservation, the increase in BeWo cryosurvival shows that future work using different CLC and cryoprotectant concentrations as well as different cryoprotectants, cell types, and cryomedia could potentially yield improved stem cell viability after cryopreservation.

Assisted the veterinarian, Dr. Carol Stevenson, in caring for the animals at her clinic._______________________________________________________-Centrifuged blood work for plasma separation and analysis-Wrapped and autoclaved surgery packs-Recorded vital signs during surgery -Documented, filed, and scanned patient history-Prepared vaccines, performed heartworm and fecal tests-Gained experience communicating and ensuring client satisfaction