Spencer Hebert Email and Phone Number

• Served as principal investigator for Biomarker Assays with experience developing and validating ICS assays, multicolor phenotyping assays, RO assays, and ELISpot assays.• Designed, developed, and qualified 17 and 18-color T cell phenotyping assays on the Cytek Northern Lights.• Highly skilled in the use of the BD FACS Canto II, BD FACS Lyric, and Cytek Northern Lights with experience analyzing flow cytometry data in FACS Diva and FlowJo.• Meticulously analyze and review development and validation data and prepare presentations to effectively communicate findings to clients.• Authored validation plans, methods, sample analysis plans, and Standard Operating Procedures.• Submit appropriate documentation to QC and QA, to include assay data, notebooks, benchsheets, and data tables.

• Serve as principal investigator for Biomarker Assays with experience developing and validating ICS assays, multicolor phenotyping assays, RO assays, and ELISpot assays.• Highly skilled in the use of the BD FACS Canto II and BD FACS Lyric with experience analyzing flow cytometry data in FACS Diva and FlowJo.• Meticulously analyze and review development and validation data and prepare presentations to effectively communicate findings to clients.• Authored validation plans, methods, sample analysis plans, and Standard Operating Procedures.• Submit appropriate documentation to QC and QA, to include assay data, notebooks, benchsheets, and data tables

• Pivotal in designing, developing, and validating fit-for-purpose eight-color flow cytometry assays in support of complex clinical protocols for key clientele. • Validated a variety of flow cytometry assays, including Natural Killer and T cell panels to detect activation, inhibition, proliferation subsets.• Regularly managed two validations simultaneously, pivoting seamlessly between differentiated requirements associated with each project.• Refined two flow cytometry assays, saving the client time and overhead costs, and enabling them to meet challenging study start deadlines.• Garnered extensive experience in the activation of different cell types, such as PBMC’s and NK cells.• Perform routine cell culture and follow BSL-2 protocols.

• Precisely facilitated validations and sample analysis of client supplied methods to measure pharmaceutical compounds both quantitatively and qualitatively, maintaining sample analysis passing criteria of 90%+. • Upheld Good Laboratory Practices and CLIA regulations related to assays. • Functioned as appointed mentor to new analysts, strengthening staff understanding of departmental standards, safety policies, and functional procedures. • Intricately reviewed and compiled results, performing data comparison against SOP acceptance criteria, methodology, protocol, and product specifications prior to QC submission.

• Aided development, validation, and production of client-supplied methods, performing compounded sample preparation with purpose of quantitatively and qualitatively measuring pharmaceutical and biopharmaceutical compounds, antibodies, and antisense oligonucleotides in varying matrices, utilizing a range of methodologies. • Organized and examined large sums of data and troubleshot procedural issues impacting assay process.

Facilitated routine cell culture protocols, cytotoxicity assays, and integrated data analysis. Coached new students on GLP standards, routine cell culture protocols, and cytotoxicity assay processes. Drafted Grant application, receiving funding through Florida Atlantic University’s Office of Undergraduate and Research Inquiry.