Sridevi Ranganathan Email & Phone Number

Toronto, Ontario, Canada

Toronto, Ontario, Canada

Toronto, Ontario, Canada

Toronto, Ontario, Canada

Toronto, Ontario, Canada

Toronto, Ontario, Canada

Baltimore, Maryland Area

I worked as a postdoctoral research fellow in Dr. Eileen Barry’s lab at the Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore. Investigating the utility of the human enteroid as a preclinical model to predict vaccine reactogenicity• Led the effort to transfer knowledge about human enteroid propagation and maintenance from collaborator’s lab to host institution.• Trained colleagues in techniques required to work with human enteroids.• Established the basic parameters of Shigella infection in the human enteroid model (Details of this work are published in Ranganathan et al, 2019. Infection and Immunity. PMID: 30642900)• Presented work at local and national meetings.Techniques used: Cell culture, organoid culture, host-pathogen interaction assays, ELISA, Western Blotting, qRT-PCR, confocal microscopy, molecular cloning techniques

Baltimore, Maryland Area

I volunteered as a CTFP fellow at Baltimore City Community College. The Collaborative Teaching Fellows Program (CTFP) gives trainees hands-on teaching experience with mentorship and coaching from exemplary undergraduate teaching faculty at local institutions in Maryland.• I supported the lead faculty in setting up and conducting the Microbiology lab course (~3 hours/week)• Mentored ~20 undergraduate students in understanding concepts and performing lab experiments

Albany, New York Area

During this short term fellowship, I worked in the NYS Clinical Virology lab, under the mentorship of Dr. Kirsten St.George and Dr. Patrick Bryant. My project focused on whole genome sequencing and de novo assembly of Norovirus strains isolated from patients. Responsibilities:• Optimizing diagnostic qRT-PCR based tests for detection and typing of enteroviruses• Understanding and training in the clinical laboratory workflow and practices• Isolation and enrichment of viral nucleic acids• Introduction to de novo genome assembly softwaresTechniques used: Introduction to Geneious WGS analysis software, qRT PCR, viral nucleic acid extraction, Clinical laboratory workflow

Albany, New York Area

I earned my Ph.D. in the field of Biomedical Sciences in the lab of Dr. Kathleen McDonough. My dissertation research focused on characterizing a novel cAMP-responsive transcription factor, Cmr, in TB complex mycobacteria. Project 1:• Investigated the function of cyclic AMP responsive transcription factor, Cmr in Mycobacterium tuberculosis (Mtb).• Identified global Cmr binding sites on Mtb genome using ChIP-seq by active collaboration with bioinformatics experts.• Validated a subset of binding using in vitro DNA-protein binding assays such as electromobility shift assays.• Established Cmr as a regulator of dormancy regulon in Mtb known to be important for establishing latent TB infection.Details of the above work are published in Ranganathan et al, 2016, Nucleic Acids Research. PMID: 26358810Project 2:• Actively collaborated with structural biologists to solve the X-ray crystal structure of Cmr• Identified key residues involved in DNA binding. Successfully mutated key DNA binding residues using molecular cloning techniques.• Established and optimized overexpression and purification of several mutant versions of Cmr protein and showed consequent loss/gain of DNA binding activity.• Used bioinformatics tools (BEDTOOLS suite) and basic programming skills (Python) to analyze large number of DNA binding sites and identified critical nucleotides that drive binding differences between two closely related transcription factors, Cmr and CRP.• Established Cmr as an atypical member of the CRP-FNR family of transcription factors.Details of the above work are published in Ranganathan et al, 2018, Nucleic Acids Research. PMID: 29165665Techniques used: Molecular cloning, nucleic acid (DNA/RNA) extraction, protein expression and purification, microbiology techniques, electromobility shift assays, ChIP-seq data analysis and validation, qRT-PCR, Western blotting, Handling pathogens in BSL-2 and BSL-3 facilities, radiation safety training

Bengaluru Area, India

I worked as an undergraduate researcher in Dr. Sudhir Krishna's lab at NCBS, Bangalore. During this time, I assisted in a project investigating Notch signaling pathway in cervical cancer.Techniques used: Cell culture, SDS PAGE electrophoresis, Western Blotting

Rajasthan, India

I worked as an undergraduate researcher learning molecular cloning techniques under the guidance of Dr. Ashis Das and Dr. Narayan Jalan at BITS, Pilani.• Assisted in a project to characterize temperature inducible promoters. • Contributed to data generation published as research article (PMID: 22192842)Techniques used: Molecular cloning, Microbiology, PCR, Aseptic technique

Mysuru Area, India

I worked in the laboratory of Dr. A. Jayadeep at CFTRI, Mysore during the summer of 2006. Project: Value addition to rice by biotransformation• Evaluated the nutritional characteristics of biotransformed rice using biochemical tests.• Drafted the results into a report and presented data collected at institutional research meeting.Techniques used: Biochemical assays

Graduate Diploma, Phmi, Pharmaceutical And Healthcare Management And Innovation

Doctor Of Philosophy - Phd, Biomedical Sciences, General

Activities and Societies: Secretary (2012-2014)- American Society for Microbiology Student Chapter (Eastern NY Regional Branch)

Post-Graduate Diploma, Immunotechnology

Master Of Science - Ms, Biology/Biological Sciences, General