Stefan Krimmer Email and Phone Number
Stefan Krimmer work email
- Valid
Stefan Krimmer personal email
I am a passionate drug discovery researcher with 7 years of post-graduate experience in protein production, X-ray crystallographic and biophysical characterization of protein–inhibitor complexes (40+ PDB depositions), and structure-function analysis of membrane and soluble proteins using single-particle cryo-EM (10+ maps). I am motivated to use my skills to provide actionable insights for the development of innovative therapeutics that may improve patients’ lives.My 2 most significant scientific achievements are:● Determining the cryo-EM structure of the full-length transmembrane receptor tyrosine kinase KIT (PNAS 2023), a task previously accomplished for only 4 other full-length RTKs. This structure revealed mechanistic insights into oncogenic activation, and a novel target for therapeutic intervention against KIT-driven diseases and cancers (patented).● Collaborating cross-functionally, I worked on the structure-based design of small-molecule ligands that bind highly selective and with high affinity to the pseudokinase domain of JAK2, a key intracellular signaling mediator (J. Med. Chem. 2020). I used these ligands as probes to test the hypothesis whether the aberrant activity of the JAK2 V617F mutant can be modulated by selectively displacing ATP from the pseudokinase domain of JAK2.I have a track record of repeatedly bringing challenging projects to completion, both as project leader and as collaborator within multi-disciplinary teams (9 first-authored and 14 co-authored publications). I am committed to excellence, recognized by a competitive PhD thesis award and a 2-year postdoctoral fellowship. I believe that I am an empathetic leader and mentor with a strong sense of fairness. As a lifelong learner, I enjoy exploring innovative technologies to solve intricate problems and collaborating with others, learning from each other’s expertise. I am committed to clear and effective communication, exemplified by giving talks at 8 scientific conferences, including 2 American Chemical Society (ACS) meetings.Let's connect if we share similar interests!
-
Cryo-Em ScientistNew York Structural Biology CenterNew York, Ny, Us -
Protein Science Group ManagerNexomics BiosciencesNew York, Ny, Us -
Postdoctoral Researcher Then Associate Research ScientistYale University Sep 2020 - PresentDepartment Of Pharmacology, Laboratory Of Dr. J. SchlessingerTeaming up with a fellow postdoc, I used cryo-EM to determine the structures of wild-type and 2 oncogenic mutants of the full-length receptor tyrosine kinase KIT in complex with its ligand SCF. These cryo-EM structures reveal molecular-level insights into the activation mechanism of KIT and its oncogenic activation, unveiling a novel target for therapeutic intervention against KIT-driven cancers and diseases. Specifically, I have:● Collaborated closely with a cryo-EM expert lab to gain hands-on cryo-EM training. Introduced this technique to our lab, training colleagues in all aspects of cryo-EM.● Proficiently designed, cloned, expressed, and purified various constructs of full-length and truncated RTKs (KIT, ALK), cytokines (SCF, METRNL, AUG-α, AUG-β), and signaling pathway proteins (PLCγ-1, PI3Kα) from bacterial, insect, and mammalian cell expression systems.● Successfully reconstituted detergent-solubilized membrane protein complexes using various stabilizing agents, such as amphipols, and peptidiscs. Identified optimal conditions for preparing grids of soluble and membrane proteins for negative staining EM and cryo-EM.● Held “superuser” status operating electron microscopes (Tecnai T12, Glacios) for grid screening and data collection using SerialEM software. Processed data using cryoSPARC and RELION software and deposited 4 maps in the EMDB.● Generated stable cell lines expressing KIT mutants and assessed their signaling activity by Western blot, establishing a functional correlation with cryo-EM structural observations.● Published 1 co-first authored paper in PNAS, and 1 co-authored paper in Nature. Delivered an oral presentation of my research findings at the 9th New England Cryo-EM Symposium (>140 participants), receiving positive feedback from expert attendees. -
Postdoctoral Researcher Then Postdoctoral FellowYale University May 2017 - Aug 2020Department Of Chemistry, Laboratory Of Dr. W. L. JorgensenCollaborating cross-functionally with synthetic and computational chemists, I worked on the structure-based design of small-molecule ligands selective for the pseudokinase (PK) domain of JAK2 kinase, a key initiator of intracellular signaling involved in cancers and other diseases. At the time, the PK domain was considered a potential innovative anti-cancer target. Furthermore, I collaborated on the structure-based design of MIF inhibitors. Our efforts led to nanomolar binders against both JAK2 PK and MIF. Specifically, I have:● Crafted a comprehensive research project proposal focused on the structure-based design of JAK2 PK ligands, securing a 2-year postdoctoral fellowship award from the German Research Foundation (DFG) totaling $109,000 in funds.● Performed protein expression and purification using bacterial, insect, and mammalian cell systems. Applied a range of purification techniques, ensuring high-quality protein for biophysical measurements and protein crystallization.● Optimized crystallization conditions and introduced a co-crystallization protocol. Independently collected X-ray data in-house and at the synchrotron and solved and interpreted 20+ crystal structures of protein–ligand complexes. The gained structural insights were essential for the rational design of potent binders.● Established and performed the Microscale thermophoresis (MST) assay to determine the affinities of small-molecule ligands binding to the truncated pseudokinase domain of JAK2. The assay provided robust binding data, facilitating the design of highly potent binders. ● Established and conducted an in vitro [γ-32P]ATP kinase assay to evaluate the enzymatic activity of full-length JAK2. This functional assay played a crucial role in testing the hypothesis that the kinase activity of JAK2 can be modulated by selective ATP displacement from the pseudokinase domain using small-molecule ligands.● Published 1 co-first authored paper and 6 co-authored papers.
-
Graduate Student ResearcherThe Philipp University Of Marburg Aug 2011 - Apr 2017Deptartment Of Pharmaceutical Chemistry, Laboratory Of Dr. G. KlebeIn close collaboration with a synthetic chemist, I investigated the impact of water molecules on protein-inhibitor binding through the analysis of kinetic, thermodynamic, and structural data of a congeneric series of small-molecule inhibitors binding to a metalloprotease. The obtained insights provide a rather general concept for how to rationally enhance ligand affinity. Furthermore, I engaged in a fragment-based screening campaign. Specifically, I have:● Independently characterized the structure-activity relationship of 20 small-molecule inhibitors using X-ray crystallography and isothermal titration calorimetry (ITC). ● Established and maintained a collaboration with GE Healthcare Life Sciences, focusing on measuring the binding kinetics of small-molecule inhibitors using surface plasmon resonance (SPR). ● Identified a poorly hydrated protein pocket by Xenon derivatization and MAD phasing, revealing its role in achieving an 41,000-fold affinity boost upon filling with an isobutyl group. This finding presents a promising drug design strategy.● Participated in a 13-member team that performed high-throughput screening of a 361-entry fragment library against an aspartic protease using crystallography. This approach led to the identification of 71 hits, 31 of which were undetected by other screening techniques, underscoring crystallography as a primary fragment screening technique in early stages of drug discovery.● Published 5 co-first authored and 7 co-authored papers.● Received the 2018 PhD Award in Medicinal/Pharmaceutical Chemistry from the German Chemical Society, recognized for an outstanding dissertation that made significant contributions to the field.● Presented my research at 6 national and international conferences, including the DiPIA conference in 2012 held in Beijing, China, for which I was awarded a scholarship from the German Academic Exchange Service (DAAD) to attend and present my work. -
Teaching Assistant In Pharmaceutical ChemistryThe Philipp University Of Marburg Aug 2011 - Apr 2017Supervised 500+ pharmacy students in practical laboratory courses focused on qualitative and quantitative drug analysis, demonstrating strong leadership, communication, and organizational skills. Conducted seminars, administered oral exams, and graded written exams, with a track record of successful student outcomes.
-
Master'S Student ResearcherUniversity Of Utah Jan 2010 - Jul 2010Department Of Pharmaceutics And Pharmaceutical Chemistry, Laboratory Of Dr. J. KopečekConducted research for my Master’s thesis in pharmacy at the University of Utah, focusing on the synthesis and characterization of block copolymer micelles for drug delivery. This work contributed to the degree awarded by Martin Luther University of Halle-Wittenberg, Germany, and resulted in 1 first-author publication.
Stefan Krimmer Skills
Stefan Krimmer Education Details
-
Summa Cum Laude (Highest Grade, In Top 12% Of Phd Graduates That Year)
-
Master'S Degree, Pharmacy -
Pharmacy
Frequently Asked Questions about Stefan Krimmer
What company does Stefan Krimmer work for?
Stefan Krimmer works for New York Structural Biology Center
What is Stefan Krimmer's role at the current company?
Stefan Krimmer's current role is Cryo-EM Scientist.
What is Stefan Krimmer's email address?
Stefan Krimmer's email address is st****@****ale.edu
What schools did Stefan Krimmer attend?
Stefan Krimmer attended The Philipp University Of Marburg, The Martin Luther University Of Halle-Wittenberg, The Philipp University Of Marburg.
What skills is Stefan Krimmer known for?
Stefan Krimmer has skills like Structure Based Drug Discovery, X Ray Crystallography, Isothermal Titration Calorimetry, Scientific Writing, Protein Expression, Protein Purification, Biochemistry, Drug Discovery, Cell Culture, Chemistry, Pharmacology, Protein Crystallization.
Who are Stefan Krimmer's colleagues?
Stefan Krimmer's colleagues are Shubhangi Agarwal, Fiana Burshteyn, Justyna Aleksandra Wojdyla, Darnell Granberry, Debbie Trifilio, Rhonda Alphonso, Sineth Kodikara.
Not the Stefan Krimmer you were looking for?
-
stefan krimmer
Zurich, Switzerland -
Stefan Krimmer
United States -
Stefan Krimmer
Gerichtlich Bestellter Berufsbetreuer - Berater Für Digitale KommunikationGreater Munich Metropolitan Area1gkk.de -
1wacker.com
Free Chrome Extension
Find emails, phones & company data instantly
Aero Online
Your AI prospecting assistant
Select data to include:
0 records × $0.02 per record
Download 750 million emails and 100 million phone numbers
Access emails and phone numbers of over 750 million business users. Instantly download verified profiles using 20+ filters, including location, job title, company, function, and industry.
Start your free trial