Stephanie Guthrie Email & Phone Number

South San Francisco, California, Us

South San Francisco, California, Us

Charlottesville, Va, Us

Crystallization and Formulation• Performed polymorphism screening for APIs and analyzed crystal properties• Developed a technique for continuous precipitation to control crystal shape and size distributions • Characterized small molecule uptake and release using fluorescence imaging and spectroscopy• Fabricated polymer/small molecule composite formulations and characterized dissolutionTechnical Development• Designed a meniscus guided coating platform to create thin films• Innovated nano- and microfluidic devices for multicomponent crystallization and microparticle synthesis• Authored proposals for beamtime, resulting in collaboration with scientists at 2 synchrotron facilities • Independently operated beamline and performed root cause analysis to troubleshoot equipment failure Established new lab• Established lab as first student under advisor and served as laboratory safety manager• Acquired equipment, developed SOPs, trained/mentored 5 graduate and 6 undergraduate students• Facilitated transport of materials to synchrotrons, managed waste removal, improved lab safety

Charlottesville, Va, Us

Vice President (2019-2020) | President (2017-2018) | Secretary (2015-2017)- Established and organized an internal department research symposium, involving poster sessions (30 posters), keynote speaker, Q&A panels and networking opportunities- Organized graduate student seminar series, drove an 100% increase in graduate student participation- Hosted 5 perspective students during recruitment (3 matriculated) and organized recruitment events- Led initiatives to build graduate student community

Charlottesville, Va, Us

Instructed and trained ~45 students on upstream and downstream bioprocessing unit operations to study green fluorescent protein (GFP) expression in recombinant E. coli Planning: Executed a seed train process in preparation for bioreactor inoculationFermentation: Trained students to use a 10L Sartorius bioreactor for aerobic E. coli fermentation - Performed sterilization, inoculation, induction, real time process monitoring and cell harvesting - Optimized growth by varying oxygen feed, agitation rate, nutrient concentration- Studied growth kinetics using the Monod model, found specific growth rate and yield parameters- Characterized oxygen transport in bioreactor using static degassing experimentsPurification: Trained students to recover and purify GPF using multiple processing techniques - Identified process improvements to enhance protein recovery during cell disruption- Explored effect of flow rate and pressure drop on protein recovery during ultrafiltration- Purified protein using hydrophobic interaction chromatography (HIC), with ÄKTA Pure HPLC- Developed a standard method for gradient elution and analyzing resolution of separationScale-up: Led students through process scaling exercise from 10L to 100L scaleIncorporated design of experiments as a course learning objective and developed lecture materialsReceived a fellowship to develop and co-instruct a new lab module on degradable crosslinked polymer synthesis and characterization (DMA, DSC, gravimetric methods).Supported critical lab functionality

Charlottesville, Va, Us

Completed laboratory inspections for all labs in the chemical engineering department at UVA to identify safety hazards. Significantly reduced EHS violations in department and prevented recurring violations. Promoted safety culture in department:- Worked with safety team to initiate safety moments at the beginning of weekly seminars- Recognized peers who demonstrated safe practices with departmental safety awards- Actively helped peers find answers to questions related to waste disposal and department policies

Ann Arbor, Michigan, Us

Fabrication and optimization of a microfluidic system for isolation of circulating tumor cells.- Optimized and validated experimental conditions to improve recovery and purity of circulating tumor cells (CTCs) from liquid blood biopsy samples- Processed >200 clinical breast cancer blood samples, used immunohistochemistry staining and fluorescence microscopy to identify and quantify CTC abundance- Expansion of CTC based cell lines from primary isolation- Fabricated PDMS and silicon/PDMS based microfluidic devices- Active member of an interdisciplinary group comprised of oncologists, clinical research coordinators, chemical engineers with variable levels or experience

Doctor Of Philosophy (Ph.D.), Chemical Engineering

Master Of Science - Ms, Chemical Engineering

Bachelor Of Science In Engineering (Bse), Chemical Engineering