Stephen Helming Email & Phone Number

Bristol, Ct, Us

Farmington, Connecticut, United States

• Provided general lab support for transgenic mouse lines and project specific support for a study of immune interactions with the vasculature in atherosclerosis models.• Performed novel cell-based endothelial cell and primary immune cell co-culture to examine endothelial cells effects on T cell response. Isolation splenic OT-I T cells and bone marrow derived antigen presenting cells, combining with transgenic endothelium.• Executed cellular biology techniques to explore T cell phenotype via cell culture and subsequent ELISA, immunoblotting, flow cytometry, western blotting.• Aided in other projects with molecular biology techniques such as qPCR, DNA isolation via mini & maxi-prep, and immunohistochemistry.• Maintained large mouse colony (13 lines, ~200 cages) of genetically engineered mouse strains through breeding, weaning, drug treatment, sample collection, and genotyping.

• Performed, interpreted and correlated complex and routine clinical laboratory tests and instruments in multiple departments independently to assist in the physician diagnosis and treatment of illness and disease.• Performed essential tests, including PCR and gram stain, and used aseptic technique in a BSL 2 clinical setting. • Proficiency in Laboratory Information systems (LIMS) i.e., EPIC, SunQuest, Clinical data entry to aid in tracking of lab results and registering patient samples.• Demonstrated ability to work independently and with a team in a high-stakes fast paced environment with integrity to deliver accurate results of clinical tests in a timely fashion.

Master'S Degree, Biomolecular Snience

Bachelor Of Science - Bs, Biomolecular Science