Stephen Ledet Email & Phone Number

Bethesda, Maryland, United States

- Conducted research under the guidance of Dr. Peter Williamson in the Translational Mycology Section, focusing on the pathophysiology and treatment of cryptococcosis.- Analyzed genomic and electronic health record (EHR) data using the All of Us Research Program database to identify genetic variants that could explain the susceptibility of previously healthy individuals to cryptococcosis.- Performed spot-plating assays to determine the identities of novel proteins in 𝘊𝘳𝘺𝘱𝘵𝘰𝘤𝘰𝘤𝘤𝘶𝘴 species that are believed to be involved in host-pathogen interactions, with hopes to further understand the disease mechanisms.- Collaborated closely with lab members and mentors, gaining experience in both wet-lab and dry-lab techniques, and developing a strong foundation in bioinformatics and microbiology research.

Chicago, Illinois, United States

The Chevrier Lab is an integrative immunology research team whose goals ultimately aim to understand the complexities of the organism’s immune response to combat infection or disease. This pursuit will hopefully uncover pieces to the puzzle in manipulating the immune system at will, revolutionizing the world of medicine and biomedical research.I spent 8 weeks with the Chevrier Lab through the Pritzker Scholar Match Program. My position on the team as an undergraduate research assistant was to catch up on the experiments my mentor and PhD student had been running before branching off on my own related project. The goals of my project fit within the tRNA “group” of the team— my experiments were specifically examining the relationship between the innate immune response and post-transcriptional modifications of certain tRNA species. My mentor also was exploring the combinatorial effects of adjuvants— I stimulated my innate immune cells with one of the single adjuvant treatments extensively studied.I performed a number of protocols and tasks in the duration of my internship. These included:- gRNA Cloning- Miniprep Purification- Bone Marrow Dendritic Cell (BMDC) Extraction from Cas9 Mice- Lentivirus Production in 293T Cells- T-Cell Extraction from OTII+ Mice- Coculture of BMDCs w/ T-Cells- Surveyor Nuclease Assay- Polysome Collection- Flow Cytometry Analysis (to assess T-Cell Proliferation)

Bethesda, Maryland, United States

Self-employed, serving as a steppingstone for youths as they master the art of algebra amongst other mathematics and head off to the classroom confident in their newfound capabilities

Reviewing and summarizing the latest literature surrounding Global Health, drawing upon connections between current topics in the field, and assisting professor with their research paper

Carefully cataloguing finances, coordinating lessons and clinics for members with pros, and attending to merchandise sales in the shop

Bethesda, Maryland, United States

Mentoring young children from ages 3 and up to not only develop their tennis abilities but also foster in them a love of the game and an active lifestyle

Bachelor'S Degree

Activities and Societies: Prototypes for Success Class of 2026 Cohort, The University of Chicago Emergency Medical Services (UCEMS) EMT-b.

High School Diploma

Activities and Societies: Varsity Tennis, Editor of Literary Magazine, Spanish Club, Investment Club, AP Biology Tutor, Member.