Steven Kass Email & Phone Number

Greater Minneapolis-St. Paul Area

• Flow cytometry QC analyst. Operate BD Fortessa, NovoCyte Quanteon, and other BD instruments with multiparametric lasers for surface and intracellular staining of target antibodies/cells.
• GMP validation and testing of products, most recently, human xhCD3.
• Experience with blocking antibodies and detection for COVID-19 (SARS-COV-2) as well as other blocking assays.
• SOP author for multiple laboratory tests.
• Mitogenic stimulation of primary cells and cell lines for differentiation.
• Differentiation of PBMCs to various immune cells including Th2, Th1, macrophages, and other immune cells.

Minneapolis, MN

• Performed complex biological assays such as mitogenic proliferation assays, mixed lymphocyte reactions (MLRs), ELISA, RIA, and ELISPOTs to monitor nonhuman primate (NHP) xenograft transplantation function in diabetes.
• ELISA assay development to evaluate islet products using IL-1β and IL-6 ELISAs.
• Flow cytometry for analyzing cell populations.
• DNA extraction and PCR analysis.
• Lab management and documentation of samples in multiple facets.
• 2nd author, Research Poster, American Transplant Congress (ATC), 2010, San Diego, CA: “Role of CD8 T Cells Activated by IL-15 in Mediating Xenograft Rejection in a Pig-to-NHP Iselt Transplantation Model”.

Minnetonka, MN

• Operated COBAS AmpliPrep/TaqMan Roche instrument for extraction and quantitation of HIV.
• Proficient in performing PCR, real-time PCR, and gel electrophoresis.
• Performed manual and automated extraction of DNA/RNA.
• Tops Award for excellence in laboratory service and practice. September 2008.

Master Of Science (Ms), Veterinary Medicine

• Applied RT-PCR-based methods, flow cytometry analysis, IHC, mitogenic assays, gross/microscopic analysis for vaccine evaluation. •.

Bachelor Of Science (Bs), Biology, General