Contribution of the progression of preclinical projects- Participate and support in CMC efforts.- Manufacturing preclinical used vectors.- Contribute analytical assays to determine quality and quantity of vectors.- Technology transfer of vector production know-how to the desired facility and CMO- Conduct the IND enabling studies- Prepare the IND submissionsDevelopment and improvement of vector platform technologies - Screen and evaluate vector producer cell lines.- Improvement of vector production downstream process.- Utilized the bioreactor to generate the vectors.Staffing and Management Function- Training the new hires to familiar with the company’s mission and culture quickly.- Supervise lab personnel to ensure the optimum performance.- Recruit qualified candidates to support Gene Therapy R&D requirements.

Setting up the vector production platform- Establish Standard Operating Procedures (SOPs)- Setup and maintain effective document and record control systems- Inventory management and sample tracking- Supervise lab personnel to ensure the optimum performanceSupporting the GLP/GMP manufacture of recombinant lentiviral vectors- Participates in CMC project team meetings- Provides requirements that pertain to CMC activities.- Assistant the GMP-grade Master Cell Banks generation- Development of column-based, downstream methods to concentrate viral vectors- Construction of the stable producer cell line to generate lentiviral vector- Optimization of production process for vector production

Setting up a lentiviral vector production platform- Establish Standard Operating Procedures (SOPs)- Setup and maintain effective document and record control systems- Inventory management and sample tracking- Supervise lab personnel to ensure the optimum performanceGeneration of stable producer cell lines for large scale vector production- Characterization of lentiviral vectors produced by stable producer lines- Generating the lentiviral vector producer cell line to deliver genetic payload to target cells- Optimization of culture condition for producer cell lines- Serum free medium and Suspension culture adaptationSupporting the GLP/GMP manufacture of recombinant lentiviral vectors- Development the therapeutical lentiviral vectors- Designing the cloning strategy and screening the vector constructs- Assistant the GMP-grade Master Cell Banks generation- Development of column-based, downstream methods to concentrate and purify the large scale virus

Evaluating the impact of immune activation on HIV disease and HCV disease patients.

While working for Dr. Pin Wang’s laboratory in USC Engineering School, I was required to design and schedule all my experiments as an independent researcher. During this process, I learned how to lead the projects, pay attention to details, and troubleshoot when the experiments fail

Manage human resources Execute army laws and rules enforcement

Operate the experimental Instrument to generate the Lithium Manganese Oxide powderComplete the project through teamwork collaboration