Steve Oliveira Email and Phone Number

• Managed three development teams within R&D: 1) Product Development; 2) Process Design; and 3) Methods Development.• Responsible for the establishment of goals for 6 high caliber full time employees.• Accountable for design of experiments (DOE), initiation of experiments, data interpretation, project timelines and budget to actual expense reports.• Responsible for review and sign-off of all project reports and documentation.

• Designed and executed experiments to generate novel self-assembled living dermal equivalent tissue constructs utilizing dermal fibroblasts, keratinocytes and mesenchymal stem cells.• Developed functional assays to elucidate angiogenic potency, immuno-modulatory, and immuno-regulatory characteristics of novel tissue constructs.• Executed Flow Cytometric Assays to characterize cell surface marker expression and cytokine/growth factor production in culture on BD FACS Canto… Show more • Designed and executed experiments to generate novel self-assembled living dermal equivalent tissue constructs utilizing dermal fibroblasts, keratinocytes and mesenchymal stem cells.• Developed functional assays to elucidate angiogenic potency, immuno-modulatory, and immuno-regulatory characteristics of novel tissue constructs.• Executed Flow Cytometric Assays to characterize cell surface marker expression and cytokine/growth factor production in culture on BD FACS Canto II.• Performed in vivo Subcutaneous and Full-thickness wound grafting in normal and nude mice to elucidate the host response to various tissue constructs.• Managed expansion and banking of mesenchymal stem cells in both monlayer and spinner flasks.• Transfection of mammalian cells by electroporation.• Extensive work in RNA isolation, RT-qPCR, biochemical assays, ELISA, western blotting, cell labeling/tracking, time lapse microscopy, and immunohistochemistry.• Created several research operating procedures (ROP) related to RNA isolation, functional assays, cell tracking, and various laboratory procedures. Show less

Received Excellence in Values & Behaviors Award 2010

- Coordinated multiple projects associated with the Metals Epidemiology Research Group.- Managed research teams (Associates, Project Coordinators, and Physicists) based in: Boston, Mexico, and Florida. - Responsible for R&D of K-X-ray Fluorescence (KXRF) bone lead analyzer medical device.- Analyzed and presented data to management obtained from independent research.- Coordinated installation of K-XRF instruments in off-site facilities and training of personnel:… Show more - Coordinated multiple projects associated with the Metals Epidemiology Research Group.- Managed research teams (Associates, Project Coordinators, and Physicists) based in: Boston, Mexico, and Florida. - Responsible for R&D of K-X-ray Fluorescence (KXRF) bone lead analyzer medical device.- Analyzed and presented data to management obtained from independent research.- Coordinated installation of K-XRF instruments in off-site facilities and training of personnel: Mexico City, Mexico; Seoul, Korea; Tallahassee, Florida - Received Partners Healthcare Award of Excellence, December 2004 Show less