➢ Worked as a study director in the GLP lab.➢ Interacted with clients for study design, Execution, support for operational challenges,and interpretation of results.➢ Performed Adventitious and Retrovirus studies for pharmaceutical products as per regulatory guidelines.➢ Designed virus clearance study in accordance with guidelines (EMA and ICH Q5A).➢ Produced and purified virus-like particles (VLPs).➢ Propagated and purified Viruses.➢ Developed and validated methods for cell-based assays and qPCR.➢ Developed and optimized the virus purification platform to enrich virus titer and stock purity using Ultracentrifugation.➢ Reviewed and interpreted raw data to produce high-quality reports and data sets suitable for inspection by regulatory agencies as per ALCOA+.➢ Prepared and implemented standard operating procedures (SOPs).➢ Managed research budgets and resources efficiently, improving project outcomes and minimizing waste.➢ Mentored junior scientists, provided training, and enhanced laboratory practices through quality control measures.➢ Managed multiple projects simultaneously, ensuring timely completion and high-quality deliverables.➢ Led a team of researchers efficiently, providing guidance and support to maximize productivity and achieve project goals.➢ Maintained a safe and organized laboratory environment by adhering to best practices for chemical handling and waste disposal.➢ Implemented quality control measures throughout the experimentation process to ensure the integrity of study outcomes.➢ Simultaneously managed several complex projects, meeting all pertinent milestones.

➢ Revived, passaged, maintained, and cryopreserved human, animal, and rodent cell lines.➢ Performed sample cytotoxicity and interference assays on cell lines.➢ Propagated viruses and enhanced the virus titer by ultracentrifugation.➢ Conducted virus titration using TCID 50 and plaque-forming assays.➢ Quantified viruses using real-time PCR.➢ Developed and validated cell-based and molecular-based assays.➢ Validated methods for mycoplasma detection.➢ Applied transfection methods to increase virus titer.

➢ Maintained the Bone Marrow Registry of HLA at Cancyte on behalf of Bangalore Medical College (BMC).➢ Isolated genomic DNA from donor samples and performed PCR for HLA typing.➢ Developed a multiplex PCR method for detecting dengue serotypes and Chikungunya virus.➢ Created a molecular detection technique for typhoid fever.➢ Developed a paper-based nucleic acid extraction technique for point-of-care diagnosis.➢ Designed serotype-specific primers for dengue detection and standardized them for conventional and real-time PCR.➢ Conducted molecular cloning.

➢ Worked on different strains of the Avian Influenza virus, which involved isolating the virus by infecting samples in embryonated eggs and identifying the viruses using Conventional PCR, Real-Time PCR, Hemagglutination (HA), Hemagglutination Inhibition (HI), and rapid antigen detection (kit).➢ Isolated and identified different animal viruses using animal cell lines and various PCR techniques (Conventional, Nested, Reverse Transcriptase, Real-Time, and Microchip-based Real-Time PCR), and cloned them into plasmids for future reference.➢ Detected Avian Influenza in grandparent stock serum samples imported from abroad.