Sudhanva Devaprasad Dixit Email & Phone Number

Boston, MA, US

Northeastern University, Boston, Massachusetts, United States

Boston, Massachusetts, United States

Boston, Massachusetts, United States

• Synthesized monodispersed GNPs of varying sizes using citrate-reduction and THPC-reduction methods
• Optimized particle stability with PEGylation to extend circulation time and reduce immunogenicity
• Formulated LNPs/liposomes using thin-film hydration and microfluidic assembly to incorporate Talazoparib and evaluated loading efficiency of the drug through HPLC
• Assessed particle size, surface charge, and stability through DLS and Zeta Potential measurements, and verified particle concentration using UV-Vis Spectroscopy
• Performed Immunocytochemistry (ICC) using anti-gH2AX and anti-Rad51 antibodies to visualize nanoparticle uptake in cells and to test the bioactivity of Olaparib-loaded LNPs in FKO1 cell lines
• Employed fluorescent secondary antibodies- Alexa Fluor dye and DAPI for nuclear staining to assess subcellular localization under fluorescence microscope

Boston, Massachusetts, United States

• Established a microbial colony on a plate and broth to perform E.coli transformation of pGLO via heat-shock method.
• Determined protein concentration using BCA assay and Performed Western Blot
• Validated GFP protein expression using SDS gel and Ponceau staining. Purified GFP protein via AKTA HPLC and HIC gravity column.
• Utilized CRISPR-Cas9 for editing lacZ gene in E.coli and generated growth curves using OD600 and CFU measurements.
• Cultured CHO cells and transfected them with the GFP protein-coding gene

Boston, Massachusetts, United States

• Developed and implemented social media strategies to boost engagement, managing content, and tracking feedback.
• Coordinated and collaborated with Academic Affairs and Operations to align activities with organizational goals.
• Designed posters and flyers for journal clubs and GBBA events, gathered people with similar interests in biotech

Noida, Uttar Pradesh, India

• Completed an intensive training course on Bioinformatics tools
• Performed DNA sequence alignment using NCBI BLAST to enhance the accuracy of genomic data analysis.
• Predicted protein structures with high precision using PDB, RasMol, and MMDB, contributing to structural biology research.
• Executed molecular docking studies of various drugs targeting specific proteins using AutoDock Vina, Biovia Studio, PyMol, Cobalt, and CBDock, facilitating drug discovery.
• Developed and published a research project titled 'NGS and Drug Development Pipeline for Alzheimer's Disease,' advancing the understanding and potential treatments for neurodegenerative disorders.Skills: NCBI BLAST.

Bioinformatics And Computational Biology

• Retrieved and curated DNA, RNA, and protein sequences from NCBI, enhancing the quality and accuracy of Biological databases.
• Conducted sequence alignment and comparison using BLAST and FASTA, optimizing primer design for qPCR and In-Silico PCR to improve experimental efficiency.
• Performed Multiple Sequence Alignments (MSA) with ClustalW and Clustal Omega, providing insights into evolutionary relationships and functional genomics.
• Implemented molecular docking and protein modeling with PyRx and Biovia software, advancing the development of potential drugs.Skills: NCBI, BLAST, FASTA, RAST, primer3, In-Silico PCR, ClustalW, Clustal Omega, SRA.

• Assisted in zebrafish genetics research project involving egg collection and selection, embryo stage visualization, and evaluated the impact of NaCl on embryonic development.
• Trained 50+ students for various techniques and equipment such as microbial cell culture, PCR, NanoDrop, and Gel electrophoresis.
• Analyzed imaging data using Fiji/ImageJ software to measure the morphological characteristics of zebrafish embryo
• Operated fluorescence microscope using ANO stains from Azooka Labs and conducted LAMP PCR for isothermal amplification and viral pathogen detection.
• Authored and published a research paper on the in-vivo toxicological study of zebrafish embryosGenomics Research Workshop:
• Evaluated qPCR data to quantify gene expression levels, facilitating the identification of key regulatory genes.

• Studied and worked on a topic in Genomic instability and cancer in-depth
• Completed the internship project on 'Expression of MDM2 oncogene in cancer cell growth and proliferation'.

Bengaluru, Karnataka, India

• Isolated DNA from plant, animal, and bacterial sources, processing over 50+ samples to achieve high-quality yields for downstream applications.
• Performed agarose gel electrophoresis to analyze DNA fragment sizes and optimize separation protocols, resulting in improved resolution and clarity of bands.
• Utilized centrifugation for sample preparation and plasmid isolation to achieve consistent plasmid DNA yields with high purity levels.
• Executed Southern blotting to identify specific bands of DNA sequences, and conducted SDS-PAGE and Western blotting for detailed protein analysis, confirming molecular weights and protein expressions.
• Quantified DNA to measure DNA concentrations to ensuring accuracy in subsequent restriction digestion and ligation processes.
• Conducted restriction digestion and DNA ligation to construct recombinant DNA molecules, facilitating successful gene cloning and vector insertion.

Mysore, Karnataka, India

• Completed 6 months of internship by working on a project aiming to develop a raisin herbal wine
• Determined the optimum amount of raisins, ginger, and swallow roots for wine
• Evaluated the biochemical parameters of the Raisin Herbal Wine and determined its antioxidant property by performing DPPH AssaySkills: Aseptic techniques, PDA (Potato Dextrose Agar) preparation, Yeast and bacterial.

Bengaluru, Karnataka, India

• Hands-on training: ✓ Fundamentals of qPCR and immunoblotting✓ Microbiome analysis- Tools and Practices
• Digital training: ✓ Immunobiology techniques✓ Genome editing tools and techniquesSkills: Pharmaceutical Industry, NanoDrop™ 1µL UV-Vis Spectrophotometer, Merck scepter 3.0, Millicell® DCI, SDS-PAGE, cDNA synthesis.

Mysore, Karnataka, India

• Enumeration of Microflora responsible for infections at health care centers
• Isolated and cultured the HAI causing bacteria and fungi
• Identified the microbes by observing their morphology and by conducting biochemical tests
• Analyzed the areas in the hospital where nosocomial infections can occur
• Evaluated the microbial air quality in the hospital environmentSkills: Aseptic techniques, Autoclaving, Bacterial cell culture, Fungi culture, media preperation (NA, PDA, NB), serial dilution, gram staining.

India

Project on Extremophiles and extreme environments

Master Of Science - Ms, Biotechnology, 3.79

Activities and Societies: NU Biology club, NU Biochemistry club, GBBACourses: Protein principles of Biotechnology Foundations of.

Master Of Science - Ms, Biotechnology, 3.79

Activities and Societies: NU Biology club, NU Biochemistry club, GBBA

Bachelor Of Engineering - Be, Biotechnology, 8.61

✓ Took part in the Bioquiz in 2019, 2020, and 2021, held as part of INDIABIO at the Bengaluru Tech Summit ✓ Participated in a workshop on.

Pre-University, Stem (Biology), 70%

✓ Attended the Astrophysics workshop ✓ Volunteered in the Biological field trip

School, 8.6 Cgpa

Activities and societies - Article writing | science club | Music | Painting ✓ School prefect: Accomplished leading and team-building.

School