• Synthesized monodispersed GNPs of varying sizes using citrate-reduction and THPC-reduction methods • Optimized particle stability with PEGylation to extend circulation time and reduce immunogenicity• Formulated LNPs/liposomes using thin-film hydration and microfluidic assembly to incorporate Talazoparib and evaluated loading efficiency of the drug through HPLC• Assessed particle size, surface charge, and stability through DLS and Zeta Potential measurements, and verified particle concentration using UV-Vis Spectroscopy• Performed Immunocytochemistry (ICC) using anti-gH2AX and anti-Rad51 antibodies to visualize nanoparticle uptake in cells and to test the bioactivity of Olaparib-loaded LNPs in FKO1 cell lines• Employed fluorescent secondary antibodies- Alexa Fluor dye and DAPI for nuclear staining to assess subcellular localization under fluorescence microscope• Conducted IC50 and MTS assay to evaluate cell viability and cytotoxic effects of nanoparticles on W0069 immortal cell lines, determining concentration thresholds for therapeutic safety and efficacy• Evaluated long-term cell survival and nanoparticle-induced cytotoxicity with Talazoparib-loaded LNPs and GNPs using the Clonogenic Assay

• Established a microbial colony on a plate and broth to perform E.coli transformation of pGLO via heat-shock method.• Determined protein concentration using BCA assay and Performed Western Blot• Validated GFP protein expression using SDS gel and Ponceau staining. Purified GFP protein via AKTA HPLC and HIC gravity column.• Utilized CRISPR-Cas9 for editing lacZ gene in E.coli and generated growth curves using OD600 and CFU measurements.• Cultured CHO cells and transfected them with the GFP protein-coding gene

• Developed and implemented social media strategies to boost engagement, managing content, and tracking feedback. • Coordinated and collaborated with Academic Affairs and Operations to align activities with organizational goals.• Designed posters and flyers for journal clubs and GBBA events, gathered people with similar interests in biotech

• Completed an intensive training course on Bioinformatics tools• Performed DNA sequence alignment using NCBI BLAST to enhance the accuracy of genomic data analysis.• Predicted protein structures with high precision using PDB, RasMol, and MMDB, contributing to structural biology research.• Executed molecular docking studies of various drugs targeting specific proteins using AutoDock Vina, Biovia Studio, PyMol, Cobalt, and CBDock, facilitating drug discovery.• Developed and published a research project titled 'NGS and Drug Development Pipeline for Alzheimer's Disease,' advancing the understanding and potential treatments for neurodegenerative disorders.Skills: NCBI BLAST, PDB, RasMol, MMDB, AutoDock Vina, Biovia Studio, PyMol, Cobalt, CBDock

• Retrieved and curated DNA, RNA, and protein sequences from NCBI, enhancing the quality and accuracy of Biological databases.• Conducted sequence alignment and comparison using BLAST and FASTA, optimizing primer design for qPCR and In-Silico PCR to improve experimental efficiency.• Performed Multiple Sequence Alignments (MSA) with ClustalW and Clustal Omega, providing insights into evolutionary relationships and functional genomics.• Implemented molecular docking and protein modeling with PyRx and Biovia software, advancing the development of potential drugs.Skills: NCBI, BLAST, FASTA, RAST, primer3, In-Silico PCR, ClustalW, Clustal Omega, SRA tool, ApE software, galaxy.org, FASTQC, SwissADME, PyRx, Biovia software

• Assisted in zebrafish genetics research project involving egg collection and selection, embryo stage visualization, and evaluated the impact of NaCl on embryonic development.• Trained 50+ students for various techniques and equipment such as microbial cell culture, PCR, NanoDrop, and Gel electrophoresis.• Analyzed imaging data using Fiji/ImageJ software to measure the morphological characteristics of zebrafish embryo• Operated fluorescence microscope using ANO stains from Azooka Labs and conducted LAMP PCR for isothermal amplification and viral pathogen detection.• Authored and published a research paper on the in-vivo toxicological study of zebrafish embryosGenomics Research Workshop:• Evaluated qPCR data to quantify gene expression levels, facilitating the identification of key regulatory genes.• Executed quality control for NGS data using FASTQC and the SRA tool to ensure high standards for genomic data integrity.3D Bioprinting workshop:• Utilized hydrogel inks and endothelial cells to fabricate tissue-like structures, gaining hands-on experience in 3D bioprinting• Developed comprehensive insights into various 3D bioprinting technologies, biomaterial selection, and bioinks.• Analyzed functional qualifiers and reviewed case studies for the improvements in bioprinting techniques.Drug Discovery and CRISPR Cas 9 gene editing course:• Participated in the project involving the drug discovery pipeline for cystic fibrosis, and supported the target identification, drug validation, High throughput screening (HTS), and lead optimization.• Applied CRISPR-Cas9 gene editing techniques for ex vivo and in vivo gene therapy, focusing on HDR and NHEJ pathways.• Designed guide RNAs using Broad Institute's CRISPRko tool, GenScript’s GenCRISPR Synthetic sgRNA design tool, IDT’s CRISPR-Cas9 guide RNA design checker, Synthego’s CRISPR Design tool, and ThermoFisher’s TrueGuide Synthetic gRNA tool to enhance precision and efficiency in genome editing.

• Studied and worked on a topic in Genomic instability and cancer in-depth• Completed the internship project on 'Expression of MDM2 oncogene in cancer cell growth and proliferation'.

• Isolated DNA from plant, animal, and bacterial sources, processing over 50+ samples to achieve high-quality yields for downstream applications.• Performed agarose gel electrophoresis to analyze DNA fragment sizes and optimize separation protocols, resulting in improved resolution and clarity of bands.• Utilized centrifugation for sample preparation and plasmid isolation to achieve consistent plasmid DNA yields with high purity levels.• Executed Southern blotting to identify specific bands of DNA sequences, and conducted SDS-PAGE and Western blotting for detailed protein analysis, confirming molecular weights and protein expressions.• Quantified DNA to measure DNA concentrations to ensuring accuracy in subsequent restriction digestion and ligation processes.• Conducted restriction digestion and DNA ligation to construct recombinant DNA molecules, facilitating successful gene cloning and vector insertion.• Mastered Polymerase Chain Reaction (PCR) to amplify target DNA sequences, leading to successful amplification and verification of gene constructs.• Performed Minimum Inhibitory Concentration (MIC) studies to assess antimicrobial efficacy, contributing to the evaluation of genetic modifications and drug resistance.Skills: DNA isolation from plant, animal and bacterial sources, Agarose gel electrophoresis, Centrifugation, plasmid isolation, southern blotting, SDS-PAGE, western blotting, DNA quantification, restriction digestion, DNA ligation, Biotransformation, Polymerase Chain Reaction (PCR), and MIC Studies

• Completed 6 months of internship by working on a project aiming to develop a raisin herbal wine• Determined the optimum amount of raisins, ginger, and swallow roots for wine• Evaluated the biochemical parameters of the Raisin Herbal Wine and determined its antioxidant property by performing DPPH AssaySkills: Aseptic techniques, PDA (Potato Dextrose Agar) preparation, Yeast and bacterial culture, usage of Brix refractometer and viscometer, Yeast cell count, determining sugar content, pH, titratable acidity, and alcohol percentage, Lab-scale fermentation, and racking

• Hands-on training : ✓ Fundamentals of qPCR and immunoblotting✓ Microbiome analysis- Tools and Practices• Digital training : ✓ Immunobiology techniques✓ Genome editing tools and techniquesSkills: Pharmaceutical Industry, NanoDrop™ 1µL UV-Vis Spectrophotometer, Merck scepter 3.0, Millicell® DCI, SDS-PAGE, cDNA synthesis, qPCR, Protein extraction, Immunoblotting, ELISA, Multiplexers, SNAP i.d. 2.0, Genome Editing, RNAi, CRISPR Cas-9, ZFNs, TALENs, Microbiome Analysis

Enumeration of Microflora responsible for infections at health care centers• Isolated and cultured the HAI causing bacteria and fungi• Identified the microbes by observing their morphology and by conducting biochemical tests• Analyzed the areas in the hospital where nosocomial infections can occur• Evaluated the microbial air quality in the hospital environmentSkills: Aseptic techniques, Autoclaving, Bacterial cell culture, Fungi culture, media preperation (NA, PDA, NB), serial dilution, gram staining, biochemical tests, MPN test, colony count, air sampling

Project on Extremophiles and extreme environments