Sudheendra Kumar Kodipalli Email & Phone Number

Bengaluru, Karnataka, India

Leading USP, DSP and Analytical team.

Goa, India

• To manage the DSP team to support R&D projects.• To conduct downstream process development for New Biological Entities (NBEs) and API- converting enzymes.• Responsible for scale-up and technology transfer to the pilot plant.• Preparation of Method development reports, Maintenance of downstream equipment and Coordinating with other team leads for process qualification.Key Accomplishments:• Optimized downstream process of an NBE to achieve purity of > 99.5% and successfully carried out process transfer to pilot scale.• Screened various reducing agents to maintain protein in the monomeric form during the purification stage and optimized the sulfitolysis process, which improved yield and purity.• Optimized Single step Chromatography method to purify API- converting enzymes with the HPLC purity of ~ 90%.• Screened various resins and optimized final polishing steps to reduce the Met impurity from 3% to 0.3% in the final Drug Substance.• Enhanced stability of API-converting enzyme by screening various formulation recipes.

Bengaluru, Karnataka

• Optimizing screening conditions to identify leads for detergent applications. • To carry out high-throughput assays using liquid handlers to identify stable variants. • Conducting Accelerated and Long-term stability studies using purified proteins to select the best molecules. • To carry out protein characterization experiments to understand failure mechanisms. Key Accomplishments: • Reviewed available quantification methods to address inconsistency in Protein quantification and purity measurement.• Developed RP-HPLC method for both quantification and purity analysis of purified protein. The novel method enabled a significant reduction in analysis per sample to the tune of 5 min. and provided both purity & quantity.

Wargal, Hyderabad

• Validated Analytical Methods for Natural Sweetener project.• Performed Certificate of analysis for in-process and finished products. • Coordinated with the Process team to develop a QBD production process to meet Indian and European product compliance.• Conducting product shelf-life studies.• Coordinating with testing labs for safety evaluation of Food Enzymes as per EU legislation.• Validating STPs and SOPs involved in the Process.• Responsible for Lab and Resource management.

Hyderabad Area, India

Responsibilities held :• Lead Protein Purification and Quantification sub-competency in the group.• Developed large-scale and High throughput protein purification methods for various protein engineering projects.• Provided Analytical support to the group and carry out library screening to identify lead molecules.• Developed novel protein formulation recipes for the Animal Nutrition business.• Developed Extraction and Purification of proteins from competitor products.• Optimized bench-scale assays to HTS level.Key Highlights:• Established protein purification capability in the group.• Purification methods developed at DKC were published in the DuPont Global PPN newsletter.• Won “Night in Town Award” for significant contributions towards establishing a high-throughput protein quantitation method in the group.• Filed an NOI for developing novel formulation recipes for Animal nutrition applications.• Performed thermal inactivation profile of a feed Enzyme for regulatory dossier filing in Brabrand.• Delivered high-quality data in four projects simultaneously. • Developed High throughput Protein quantitation and purification methods for Library screening and shared with global sitesOther Responsibilities held:• Served as a SPOC to the FS&RE team for instrument maintenance.• Prepared SOPs and PSMs to ensure safe operations in the lab.• Trained Research assistants, colleagues and mentored interns for their academic projects.Courses/Training attended:• Attended 3-day training in “Liquid chromatography” conducted by Waters India at Hyderabad on 10th July 2013.• Attended 4-day course on “Strategy of Experimentation with Minitab” at DuPont Knowledge center, Hyderabad on 1st Feb 2013.

Bengaluru, Karnataka, India

Roles and Responsibilities:• Developed methods for expression of Recombinant viral proteins in bacterial expression hosts.• Screened clinical samples and cell culture samples for the presence of Rotavirus and Enterovirus based on RNA profile.• Determined Oligomeric nature of viral proteins using Analytical Size-Exclusion Chromatography.• Maintained patient information and screening data.• Lab management, Instrument maintenance and chemical procurement.Key highlights:• Established methods for overexpression of toxic viral proteins in various E.coli strains.• Developed purification methods for membrane-bound viral proteins.• Actively participated in the scientific discussions and presented many research papers during lab meetings.• Co-authored two publications in peer reviewed journals.

Bangalore, India

Hyderabad Area, India

Roles and Responsibilities:• Guiding academic projects in Industrial Microbiology.• Conducting practical training courses for undergraduate students.• Maintenance of stock cultures of industrially important Bacteria Fungi.Key Highlights:• Isolated and purified Amylase and Lipase from Bacillus subtilis.• Extracted and purified Bacitracin from Bacillus subtilis.• Conducted two days workshop on Techniques in Recombinant DNA technology and Genetic engineering at Siddhartha P.G Women’s college, Hyderabad.• Conducted ten days workshop on Enzymology in Bhilai Mahila Mahavidyalaya, Bhilai, Chhattisgarh.

Master Of Science (M.Sc.), Microbiology, General

M.Sc. I learned some of the important laboratory techniques which play a vital role in the career of a Microbiologist. During my period.

Bachelor Of Science (B.Sc.), Microbiology, General

Studied Microbiology as a Major and Chemistry and Zoology as minors.