Sujoy Lahiri Email and Phone Number

Lead Scientist, R&D, Cell Biology, Primary Cell Isolation, cryopreservation, and Characterization. Product Development, Verification, and Validation. Assay Development. Building Inter and Intra organizational collaborations.

R&D at Hepatic and Cell Biology. Working in Hepatic biology, 3D Hepatic Spheroids, ADME/Tox assays, Disease Modelling, and Primary Cell Isolation and Characterization.

Crop Protection Discovery: Screening and Biochemical Assays of potential herbicides in the pipeline.

Obesity, Diabetes and Cancer Research.Following were the projects I worked in the Department of Pharmacology in the University of Virginia: 1) Determining the molecular regulation of fatty acid metabolism in the liver, 2) Determining how diet and obesity drive liver cancer initiation and progression, 3) Determining whether the metabolic pathway of lipogenesis can be therapeutically targeted to prevent or treat alcohol-induced liver cancer, and 4) Identification of small molecule drugs that increase mitochondrial metabolism for the treatment of obesity and metabolic disorders. I used a wide range of in-vivo and in-vitro models in these projects. These included knock-out mice models with tissue specific genetic knockouts, assays using primary hepatocytes, cancer and non-cancer cell lines and metabolic and immunoassays using serum and plasma. The techniques involved were metabolic assays using various radionucleotides, cellular oxidative capacity using SeaHorse XF analyzers, ELISA, Immunoblots, Immunohistochemistry, Real-time PCR, 2D and 3D cell cultures.

With my expertise in Lipid Biochemistry and Cell & Molecular Biology, at the NIH, I have studied the mechanism of non-vesicular lipid trafficking between intracellular organelles. I used the strength of yeast genetics using S. cerevisiae as the model organism and worked extensively on genetics, biochemistry, microscopy and cell biology.

Undertaken teaching courses on “Lipid metabolism in Physiology and Disease” for the students in the Foundation for Advanced Education in the Sciences (FAES) at the NIH in spring 2013 and 2014 (http://faes.org/course-catalog#n BIOC527). Responsibilities included designing syllabus, preparing course materials and delivering lectures for the students and scientists studying or working under the federal government.

Worked in the lab of Tony Futerman for 6 years in a tremendously energetic and enthusiastic group. Specialized in lipid and protein biochemistry and enzyme kinetics. Discovered the first mammalian synthase. For the first time performed detailed biochemical characterization of all six mammalian ceramide synthase. Discovered FTY720 to be a novel inhibitor of ceramide synthase and characterized the mode of inhibitory action. Developed biochemical assays for analyzing neutral lipid composition of mouse liver.

Coordinated and delivered theoretical courses of Human Physiology and supervised laboratory training courses of Biochemistry, Experimental Physiology and Histology under the curriculum of University of Calcutta to undergraduate students of honors and general discipline.