Sumathi Jayakumar Email & Phone Number

Emeryville, California, US

• mRNA-based immuno-oncology and immuno-therapy products using SAM-RNA and lipid-nano-particles (LNP) technology across phase I and phase II clinical trials. Successfully delivered 11 product lines for off-the-shelf and.
• SME for SAM-RNA drug-substance manufacturing.

Pleasanton, California, US

• Perform stability studies, develop and optimize assays for new projects
• Validation and verification of new testing protocols
• Manage laboratory workflows, inventory and supplies

Sunnyvale, CA, US

• Study adenosine A2A receptor (a G-protein coupled receptor - GPCR) in curing skin diseases
• Perform cAMP assay using CHO cell lines to study efficacy of small molecule compounds
• Data analysis using Excel and GraphPad Prism
• Manage laboratory supply inventory, order supplies and general maintenance of laboratory

Menlo Park, CA, US

• Sample analysis using ELISA for sRICA assay
• RNA isolation from human tissue and cDNA analysis, sample preparation for qPCR
• Data analysis using Excel
• Managing laboratory supply inventory, ordering supplies and general maintenance of laboratory

Rahway, New Jersey, US

• RNA isolation from various tissue samples e.g. spleen, lymph node, small intestine, colon, blood
• Quality analysis of RNA using Agilent Bioanalyzer
• Document projects using an Electronic Lab Notebook (ELN)

South San Francisco, CA, US

• Design, execute and optimize assays using data analysis software
• Produce HLA typing kits using ABI 7900, QuantStudioTM 6 and Roche platforms
• Perform and troubleshoot layout, buffer, plate and sample QC experiments using PCR/qPCR
• Follow ISO compliant work instructions and Standard Operating Procedures (SOPs). Maintain detailed records and documentation (batch and production records) as per Current Good Manufacturing Practices and Current Good.
• Mentoring and training junior associates on various aspects of the process

US

• Responsibilities included constructing genomic library resources for distribution to the scientific community for further research. Bacterial Artificial Chromosome (BAC) libraries were used for human-genome sequencing.
• Library Screening. Performed hybridization using radio labeled synthetic Oligonucleotide probes.
• Quality-Assurance of Knockout Mouse Models. Performed Gateway reactions, PCR and prepared Glycerol stocks for further research.
• Standardize Fosmid Library Pool Strategies. Standardization of pool strategies to construct Pine Fosmid libraries. Performed serial dilution of the harvested cells at different concentrations to determine optimal pool.
• Picking Individual clones. Responsible for accurately streaking and picking specific clones from various libraries as LB STAB cultures (200 clones/day).
• Characterization of BAC-clones. Performed restriction enzyme digestion and Pulse Field Gel Electrophoresis (PFGE) of digested BAC DNA to study average DNA insert size.

Education record

Open University, Biotechnology

Master Of Philosophy (M.Phil.), Biochemistry