Allen Sun Email & Phone Number

New York City Metropolitan Area

• Gene to protein construct design and cloning for high resolution structural determination of protein complexes using X-Ray crystallography and Electron Microscopy technologies
• Protein Production in bacterial, insect, or mammalian cell expression systems
• Protein Purification using multiple SEC and Affinity column strategies
• Utilize current molecular biology techniques to generate recombinant DNA
• Maintain long-term cell cultures and general lab maintenance

New York City Metropolitan Area

• Generate and develop novel methods for producing Adeno-Associated Virus (AAV) vectors and accurately evaluate their respective concentrations
• Utilize current molecular biology techniques to generate recombinant DNA
• Maintain long-term cell cultures
• Utilize state-of-the-art laboratory based machinery and computer software
• Produce recombinant Adeno-Associated Virus (rAAV) for use in gene therapy studies
• Coordinate rAAV production for use by institutional and… Show more

New York, New York

• Project: To examine the effects of ephrin-Eph cellular signaling in prostate epithelial tumorigenesis
• Designed CRISPR genome editing strategy to knockout EphA2 in prostate epithelial cell lines, bacterial transformation, sequence verification
• Screened and identified receptor - ligand expression levels in malignant and normal prostate cell lines, BCA Assay, Western Blot
• EphA2 siRNA gene silencing to explore phenotypic effects in cellular signaling and… Show more Project: To examine the effects of ephrin-Eph cellular signaling in prostate epithelial tumorigenesis
• EphA2 siRNA gene silencing to explore phenotypic effects in cellular signaling and differentiation, qRT-PCR, Western Blot, Migration Assay
• Managed, maintained, and organized lab reagents and consumables to facilitate research projects

Ridgefield, CT

• Design and execute molecular biology experiments for multi-specific biotherapeutic constructs; high-throughput sequence analysis; protein expression and characterization of engineered constructs
• Validate and implement phage display technologies for engineered biologics; library screening and assay development
• Virtual cloning, primer design, and map creation with sequence editing and annotation software (Seqbuilder, Vector NTI)

Cranberry, New Jersey

• Phage Display, Selection, and Characterizationo Performed library screening and construction in scFv/Fab format using phage displayo Characterization of biologics using Octet/Biocore biophysical techniqueso Application.
• Developed the design and optimization of cell based assays; cell culture
• Focused on established and novel science and… Show more
• Focused on established and novel science and technology trends to meet project needs
• Supported publication, patenting of scientific work, business development activities, and solutions for innovation and collaboration Show less

South San Francisco

• Developed Humaneered Antibodies Against Infectious Disease, Autoimmunity, and Cancer o Determined anitgen binding format and optimal concentration of reference chimeric Fab o Compared affinity measurements between.
• Developed Humaneered Antibodies Against Infectious Disease, Autoimmunity, and Cancer o Determined anitgen binding format and optimal concentration of reference chimeric Fab o Compared affinity measurements between.
• Developed Signal-Less Secretion System of Humaneered Fab’s o Molecular cloning of variable regions into signal-less expression vectors o Expression studies using bacterial strains for suppressors of signal sequence.
• Design, Develop, and Implement New Antibody Humanizing Technology o Molecular cloning of Fab variable regions into expression vectors o Cassette library cloning of antibody frameworks using human and murine sequences.

Richmond, CA

• Armed Virus Program for Intratumoral Infection and Lysis: Developed selectively replicating adenoviral vectors expressing Interleukin-2 in early and late stage infection cycles for the treatment of a range of cancerso.
• Armed Virus Program for Intratumoral Infection and Lysis: Developed selectively replicating adenoviral vectors expressing Interleukin-2 in early and late stage infection cycles for the treatment of a range of cancerso.
• Adenovirus Serotype Investigation into the Effective Delivery of a Selectively Replicating Systemic Virus via Intravenous Injectiono MTT cytolytic assay of 12 different wildtype adenovirus serotypes for their inherent.