Allen Sun Email & Phone Number

New York City Metropolitan Area

• Gene to protein construct design and cloning for high resolution structural determination of protein complexes using X-Ray crystallography and Electron Microscopy technologies• Protein Production in bacterial, insect, or mammalian cell expression systems• Protein Purification using multiple SEC and Affinity column strategies• Utilize current molecular biology techniques to generate recombinant DNA• Maintain long-term cell cultures and general lab maintenance

New York City Metropolitan Area

• Generate and develop novel methods for producing Adeno-Associated Virus (AAV) vectors and accurately evaluate their respective concentrations• Utilize current molecular biology techniques to generate recombinant DNA• Maintain long-term cell cultures• Utilize state-of-the-art laboratory based machinery and computer software• Produce recombinant Adeno-Associated Virus (rAAV) for use in gene therapy studies• Coordinate rAAV production for use by institutional and… Show more • Generate and develop novel methods for producing Adeno-Associated Virus (AAV) vectors and accurately evaluate their respective concentrations• Utilize current molecular biology techniques to generate recombinant DNA• Maintain long-term cell cultures• Utilize state-of-the-art laboratory based machinery and computer software• Produce recombinant Adeno-Associated Virus (rAAV) for use in gene therapy studies• Coordinate rAAV production for use by institutional and outside collaborators• Read current scientific literature to learn new production and analytical techniques• Implement new protocols to increase rAAV production efficiency• Maintain electronic databases Show less

New York, New York

Project: To examine the effects of ephrin-Eph cellular signaling in prostate epithelial tumorigenesis• Designed CRISPR genome editing strategy to knockout EphA2 in prostate epithelial cell lines, bacterial transformation, sequence verification• Screened and identified receptor - ligand expression levels in malignant and normal prostate cell lines, BCA Assay, Western Blot• EphA2 siRNA gene silencing to explore phenotypic effects in cellular signaling and… Show more Project: To examine the effects of ephrin-Eph cellular signaling in prostate epithelial tumorigenesis• Designed CRISPR genome editing strategy to knockout EphA2 in prostate epithelial cell lines, bacterial transformation, sequence verification• Screened and identified receptor - ligand expression levels in malignant and normal prostate cell lines, BCA Assay, Western Blot• EphA2 siRNA gene silencing to explore phenotypic effects in cellular signaling and differentiation, qRT-PCR, Western Blot, Migration Assay• Managed, maintained, and organized lab reagents and consumables to facilitate research projects• Oversaw and implemented lab safety regulations Show less

Ridgefield, Ct

• Design and execute molecular biology experiments for multi-specific biotherapeutic constructs; high-throughput sequence analysis; protein expression and characterization of engineered constructs• Validate and implement phage display technologies for engineered biologics; library screening and assay development• Virtual cloning, primer design, and map creation with sequence editing and annotation software (Seqbuilder, Vector NTI)

Cranberry, New Jersey

• Phage Display, Selection, and Characterizationo Performed library screening and construction in scFv/Fab format using phage displayo Characterization of biologics using Octet/Biocore biophysical techniqueso Application of structural and computational protein engineering in the design, identification, and characterization of antibody structure and function• Developed the design and optimization of cell based assays; cell culture• Focused on established and novel science and… Show more • Phage Display, Selection, and Characterizationo Performed library screening and construction in scFv/Fab format using phage displayo Characterization of biologics using Octet/Biocore biophysical techniqueso Application of structural and computational protein engineering in the design, identification, and characterization of antibody structure and function• Developed the design and optimization of cell based assays; cell culture• Focused on established and novel science and technology trends to meet project needs• Supported publication, patenting of scientific work, business development activities, and solutions for innovation and collaboration Show less

South San Francisco

• Developed Humaneered Antibodies Against Infectious Disease, Autoimmunity, and Cancer o Determined anitgen binding format and optimal concentration of reference chimeric Fab o Compared affinity measurements between murine and chimeric Fab in ELISA and ForteBio kinetic analysis o Purified and quanitified chimeric and humaneered Fab’s via periplasmic fractionation, protein G column purification; FPLC o Coomassie staining and western… Show more • Developed Humaneered Antibodies Against Infectious Disease, Autoimmunity, and Cancer o Determined anitgen binding format and optimal concentration of reference chimeric Fab o Compared affinity measurements between murine and chimeric Fab in ELISA and ForteBio kinetic analysis o Purified and quanitified chimeric and humaneered Fab’s via periplasmic fractionation, protein G column purification; FPLC o Coomassie staining and western analysis o Determined parameters for screening of minimal essential binding specificity determinant (MEBSD) libraries o Screened high affinity binders from MESBD libraries: Colony Lift Binding Assay (CLBA) and competitive ELISA o ForteBio kinetic analysis of variable region library screens o Characterized anti-idiotype antibodies for epitope specificity of pipeline products for clinical studies• Developed Signal-Less Secretion System of Humaneered Fab’s o Molecular cloning of variable regions into signal-less expression vectors o Expression studies using bacterial strains for suppressors of signal sequence defects o Investigated optimal induction conditions for library screens: periplasmic fractionation and western analysis o Sequence alignment and verifications • Design, Develop, and Implement New Antibody Humanizing Technology o Molecular cloning of Fab variable regions into expression vectors o Cassette library cloning of antibody frameworks using human and murine sequences: overlapping PCR o Characterize binding kinetics and specificity of identified Fab bindersPOSTERBebbington, CR, Baer, M, Chang, T Flynn, P, Luehrsen, K, Martinez, D, Sun, A et. al., An Engineered Human Antibody for the Treatment of Pulmonary Pseudomonas aeruginosa Infection. KaloBios Pharmaceuticals Inc. (2006) Show less

Richmond, Ca

• Armed Virus Program for Intratumoral Infection and Lysis: Developed selectively replicating adenoviral vectors expressing Interleukin-2 in early and late stage infection cycles for the treatment of a range of cancerso Molecular cloning of gene of interest into plasmid DNA; restriction enzyme digests, restriction fragment purification, DNA enzyme ligation, and gel electrophoresis analysiso Transformation for large preparation and Qiagen Maxi Prep of recombinant plasmid DNA;… Show more • Armed Virus Program for Intratumoral Infection and Lysis: Developed selectively replicating adenoviral vectors expressing Interleukin-2 in early and late stage infection cycles for the treatment of a range of cancerso Molecular cloning of gene of interest into plasmid DNA; restriction enzyme digests, restriction fragment purification, DNA enzyme ligation, and gel electrophoresis analysiso Transformation for large preparation and Qiagen Maxi Prep of recombinant plasmid DNA; Western Blot Analysiso Co-transfection of recombinant plasmid with full-length viral Terminal Protein DNA for subsequent numerous rounds of tissue culture plaque assay--screened by PCRo Maintenance of cell/tissue culture (tumor cell lines, cell counting, passaging, and freezing,)o Cell Proliferation Assays and ELISA Assay to gauge function and level of expression of IL-2 at different time-points during infectiono MTT cytolytic assays to measure the level of cell death due from viral lysis in p53 defective and intact p53 cell lines at various Multiplicities of Infectiono Large-scale production of screened viruses for CsCl-gradient purification• Adenovirus Serotype Investigation into the Effective Delivery of a Selectively Replicating Systemic Virus via Intravenous Injectiono MTT cytolytic assay of 12 different wildtype adenovirus serotypes for their inherent infectibility on various tumor cell lines and normal cell lines o Large-scale production of adenovirus serotypes on CsCl-gradient for in vivo and in vitro studieso Spectrophotometic analysis and PicoGreen assay to quantify viral DNA for in vivo characterization: DNA/RNA isolation from whole tissue tumors and organs from intratumoral injection and intravenous injection, respectively, to investigate bio-distribution of various adenovirus serotypeso Plaque Assay for adenovirus serotypes to calculate particle counto Southern Blot analysis to gauge replication level; Western Blot to gauge viral late protein expression Show less