Provide logistical oversight and technical expertise in daily operations of core facilities in biomedical engineering departmentProvide technical, research and teaching support in cell and molecular biology, tissue engineering and biomaterials characterization• Coordinated development of new Cell Mechanics Laboratory: Selected and procured laboratory equipment and supplies required for a fully functioning cell and molecular biology research facility in a class 10,000… Show more Provide logistical oversight and technical expertise in daily operations of core facilities in biomedical engineering departmentProvide technical, research and teaching support in cell and molecular biology, tissue engineering and biomaterials characterization• Coordinated development of new Cell Mechanics Laboratory: Selected and procured laboratory equipment and supplies required for a fully functioning cell and molecular biology research facility in a class 10,000 cleanroom• Coordinated renovation/upgrade of Tissue Engineering core facility• Coordinated development of Microbiology core facility• Assisted in development of undergraduate Tissue Engineering Minor curriculum; Developed and taught two new courses in conjunction with minor (see below under teaching experience)• Assisted with development and implementation of new undergraduate course “Biomaterials Characterization Laboratory”• Developed customized protocols for commonly performed processes and analyses in core labs• Trained graduate students, undergraduate students and other lab users in operation of equipment and in general cell and molecular laboratory procedures; Assisted core facility users in development and implementation of experiments• Developed safety documents and training protocols, and assured compliance with laboratory safety and other research related regulations • Assisted faculty with proposal development and grant management• Assisted students and faculty with analysis of research results and preparation of presentations and publications• Served as department liaison with university facilities management• Assisted with teaching of “Genes to Tissues” cell and molecular biology course, offered as part of the graduate core curriculum in biomedical engineering Show less

Joint Department of Biomedical Engineering, NC State University and UNC Chapel Hill• Assisted with development and implementation of new undergraduate course “Biomaterials Characterization Laboratory”; Course coordinator, Spring Semester 2012-present• Developed undergraduate/graduate dual level survey course, “Tissue Engineering Fundamentals”; Course director and co-instructor, Spring Semester 2009-present• Developed 7-week Tissue Engineering Technologies laboratory module;… Show more Joint Department of Biomedical Engineering, NC State University and UNC Chapel Hill• Assisted with development and implementation of new undergraduate course “Biomaterials Characterization Laboratory”; Course coordinator, Spring Semester 2012-present• Developed undergraduate/graduate dual level survey course, “Tissue Engineering Fundamentals”; Course director and co-instructor, Spring Semester 2009-present• Developed 7-week Tissue Engineering Technologies laboratory module; Course director and instructor, Fall Semester 2008-present• Assisted with “Genes to Tissues”, a graduate level course in cell and molecular biology and genetics; Assistant course director and co-instructor, Fall 2005, Spring 2006Duke University, Department of Zoology• Instructor: Taught undergraduate/graduate dual level developmental biology lecture course, Spring 1994 & 1995University of North Carolina at Chapel Hill, Program for Minority Advancement in Biomolecular Sciences• Organized and taught two-week developmental biology section of a six-week intensive cell and molecular biology laboratory course, Summer 1993 &1994• Contributed to development of course manualMeredith College, Health Sciences Department• Adjunct Faculty: Taught introductory undergraduate level general biology laboratories; Spring 1991Duke University, Department of Zoology • Graduate teaching assistant: Assisted with genetics lecture course and taught cell biology and physiology laboratories; Fall1982 through Spring 1984 Show less

• Prepared Adverse Drug Event data from pharmaceutical industry clients for automated conversion to MedDRA, under contract to CodingPlus• Developed training materials for data preparation process

Managed 33-month federal contract with the Centers for Disease Control and Prevention (“Develop and Pilot Test a Process to Collect Positive Samples and Establish Stably Transformed Cell Lines Suitable for Use in PE/QA of Human Molecular Genetic Testing”, contract #200-2000-10050):• Successfully met contract objectives to develop and pilot a protocol to create critically needed positive control lymphoblast cell lines with genetic disease mutations for use in clinical molecular genetic… Show more Managed 33-month federal contract with the Centers for Disease Control and Prevention (“Develop and Pilot Test a Process to Collect Positive Samples and Establish Stably Transformed Cell Lines Suitable for Use in PE/QA of Human Molecular Genetic Testing”, contract #200-2000-10050):• Successfully met contract objectives to develop and pilot a protocol to create critically needed positive control lymphoblast cell lines with genetic disease mutations for use in clinical molecular genetic testing applications• Completed 20 contract deliverable items on schedule and within budget of approximately $875,000• Directed project activities for 30 participating molecular genetics laboratories and 2 subcontractors• Planned and participated in three expert panel meetings, which included up to 12 experts in molecular genetic testing plus personnel from Duke University and the CDC• Prepared technical reports for the CDC, including 300 page final monograph• Presented project results at five national professional meetings• Published six peer reviewed articles (four first author) Show less

• Developed quantitative PCR method to monitor expression of 8 mucin genes, and showed that expression of MUC3, 4, 5AC, 5B and 6 increased during differentiation of respiratory epithelial cells grown at air-liquid interface• Supervised one technician• Used in situ hybridization to study gene expression in rat airways during embryonic lung development

• Demonstrated that the DNA regulatory gene Hox 1.3 is induced by retinoic acid in bronchial but not dermal fibroblasts, and that the protein becomes concentrated in the nucleus (Cover photo, Am. J. Resp. Cell Molec. Biol., 1992, Vol 7(1))• Described the induction of relaxin expression during squamous differentiation of rabbit tracheal epithelial cells; Cloned and characterized 5’ flanking region of gene, and identified retinoid responsive regions

• Employed digitized video microscopy and ratio imaging of fluorescent probes to show that different platelet-derived growth factor (PDGF) isoforms had different effects on intracellular calcium levels in fibroblasts and vascular smooth muscle cells.• Investigated second messenger signal transduction pathways leading to mitogenesis in vascular smooth muscle cells

Ph.D. Research; Dissertation title: “Cellular studies on early morphogenetic events in the sea urchin embryo”• Used multiple intracellular markers to show that dissociated embryonic sea urchin cells maintained internal polarity during reaggregation, and that reorganization of epithelia was an early event in formation of embryo-like structures from aggregates• Found that presumptive mesodermal cells retained their fate during differentiation of aggregates, but that the fate of… Show more Ph.D. Research; Dissertation title: “Cellular studies on early morphogenetic events in the sea urchin embryo”• Used multiple intracellular markers to show that dissociated embryonic sea urchin cells maintained internal polarity during reaggregation, and that reorganization of epithelia was an early event in formation of embryo-like structures from aggregates• Found that presumptive mesodermal cells retained their fate during differentiation of aggregates, but that the fate of presumptive ectodermal cells could be altered• Received award for Best Graduate Student Poster, 1986 Southeast Regional Developmental Biology Conference, Beaufort, NC Show less