Inventor on 10 patents and patent applicationsEstablished genome editing using crispr-Cas9, generated vectors and protocols for guide RNA synthesis, in vitro DNA cleavage assay and homologous recombination using DNA repair templates. Successfully overexpressed difficult to express heterologous proteins such as: 1. co-factor containing proteins (FAD-, copper-, heme-containing proteins)2. tetrameric membrane proteins 3. lipases/acyltransferases4. non-secreted proteins like plant chlorophyllases, pheophytinases. 5. S. pyogenes Cas9 in fungi and E. coliImproved expression of copper dependent enzymes using metallo-chaperones & small secreted cysteine rich proteins using foldases and chaperonesGenerated a secretome tool box consisting of different signal peptides derived from highly expressed secreted fungal proteinsIdentified a fusion partner for membrane protein expression Generated libraries of codon optimized heterologous proteins for lead discoveryScreened expression libraries for protein expression and enzyme activity Fermentation and screening in microtiter plate formats Built several production strains for feed/food applications and prepared regulatory documentsCollaborated with the application team in Brabrand, Denmark, China Research Center and Dupont Pioneer, Iowa

Inventor on 18 issued patentsSet-up different expression systems involving filamentous fungi, yeasts, Bacillus and StreptomycesDirectly involved in lead discovery and successfully bringing novel enzyme products (now commercially available Hexose oxidase, lipases and acyltransferase)Carried out fundamental research on identification and characterized of several transcription factors involved in carbon metabolism, gene regulation of protein secretion, and optimization of protein expression in fungi.Experience working with different EU partners on international projectsCo-principal investigator in 2 European Union funded research projects:• Filamentous fungi as cell factory for the production of proteins and fungal metabolites (1997-2000)• Designing and improving health- and food-related production processes using filamentous fungal cell factory (2000-2003)