Scott Vaccaro Email & Phone Number

Boulder, Colorado, United States

-Process Development - Purification of DNA processivity proteins to be used in Medical Device kits.-Successfully developed purification protocols for E. coli DNA Pol I, RNase Inhibitor, Klenow, T4 DNA Pol and T4 PNK.-Purification using IEX, HIC, Mixed Modal and Affinity resins on an AKTA Pure.-SDS-PAGE analysis and gel scanning to determine purity.-Fluorescent assay development to determine specific activity -Picogreen and molecular beacons to replace radioactivity assays-Took ACS 3-day course on HPLC principles Sept/Oct. 2021

Greater Salt Lake City Area

- Process Development for production of target proteins for D-Peptide inhibitor research. - Protein expression optimization using microbial platforms for production.- PCR, cloning, DNA extraction preps, bacterial transformations, SDS-PAGE gel analysis.- Protein purification (AKTA Explorer) by affinity (IMAC) and SEC chromatography.- Cell lysis by using Microfluidics for soluble protein recovery.

Greater Denver Area

Boulder Colorado

BioPlx is initially focused on control of hospital (HA) and community acquired (CA) dermal (exobiomic) disease, particularly:MRSA - (methicillin-resistant Staphylococcus aureus)v-MSSA (virulent - methicillin-susceptible Staph aureus)… with an emphasis on preventing recurrence and re-infection- Lab Supervisor- Main focus was to Introduce new genes into Staph aureus genome by heterologous recombination- DNA extractions, PCR, cloning, Western blots and inventory management

Greater St. Louis Area

New Product Development- Involved in the development of new products using recombinant protein expression technology.- Process development by microbial and eukaryotic systems. SOP development. Involvement with QC/QA.- Responsible for optimization and scale-up of recombinant proteins using animal free components - Transfer of manufacturing processes to Production Groups for pilot and engineering.runs.- Introduced and demonstrated the baculovirus expression system as a valuable research and production tool for the facility.

Boulder Colorado

Protein Expression-Crystallography- Supported the research and development of small molecule inhibitors in early drug discovery. - Provided recombinant materials for crystallography research. - Updated insect cell expression lab with Wave Bioreactors, cell counters and continuous flow centrifuge to increase output. - Increased consistency of insect cell expression by applying infection kinetics. - Obtained new cell lines and technology to increase versatility for challenging projects. - Provided cloning, purification and crystallography support as needed. - Trained several colleagues on cell culture/baculovirus expression techniques.

Master Of Business Administration (Mba) Graduated May 2013, Business Administration And Management, General, 3.83

Core classes taken to date: Management International Business Marketing Marketing Research Organizational Behavior Finance/Advanced.

Bachelor Of Applied Science (Basc), Biology

Activities and Societies: TKE / M𝜭N