Associate ScientistNov 2016 - Feb 2019▸Speaker at 2019 BEBPA US Bioassay Conference (CA, USA)•Development, optimization and troubleshooting of immunological, cell-based and neutralizing antibody assays as per client specification•Design and execution of method qualification and validation protocols•Completion of experiments following SOP, deviation and change control programs and cGMP guidelines•Preparation of technical reports, certificates of testing and analytical testing summaries

• Purified proteins using various chromatographic techniques on an FPLC system• Cloning of DNA constructs to be used in experiments using subcloning and PIPE cloning techniques• Characterized the interactions between two proteins using a variety of biochemical and cell-based techniques including: affinity pull-down assays, isothermal titration calorimetry, yeast two-hybrid assays, mammalian cell culture and macromolecular crystallography• Trained students in proper laboratory protocols

• Assessed expression and solubility of new protein constructs• Purified protein using a variety of chromatographic techniques on a fast protein liquid chromatography system• Maintained supplies, chemical solution stocks, and general cleanliness of laboratory

• Maintained a clean and safe work environment for other members of the lab • Provided training on CD Spectrometer as required• Ensured laboratory supplies were stocked • Carried out experiments for other lab members as requested

• Held 1 on 1 consultations with students, providing feedback on resumes and cover letters• Provided guidance and advice for students during mock interview process• Directed students to appropriate resources while sitting at the front desk

• Conducted literature searches to optimize the purification of novel proteins• Employed various chromatography techniques such as anion exchange, cation exchange, immobilized metal affinity, size exclusion, and mix mode chromatography on a FPLC system • Carried out fluorescence based assays on fluorometer to assess activity of newly purified enzyme• Assessed purified protein using SDS-PAGE, Western blots, and Bio blots.• Trained new lab members in the preparation of SDS-PAGE gels

• Assisted in stability studies of different products through various testing including: API liquid and solid assays using Agilent 1100/1200 series HPLC, density measurements using the Mettler Toledo DR40 Combined meter, viscosity measurements, odour and appearance testing, and dissolution testing with the Varian VK 7030 dissolution baths.• Entered data into Empower software and corporate FUSION software• Method development of a stability indicating method of an API and its degradents on HPLC and Waters Acquity UPLC• Presented at a divisional meeting and presented information about new active pharmaceutical ingredients

• Performed routine calibration of pH meters and SMART TRAC 5 machine• Ensured machines on the line were fit to start through inspection of labels, metal detectors, and areas at high risk of improper cleaning with swabs.• Obtained aseptic samples from production lines every 2 hours and tested the fat and solid compositions, acidity, and for the presence of aerobic bacteria, coliform, yeast and mold. • Approved incoming shipments of oils, chocolate, and dairy products following completing physical testing as required by company SOPs.• Updated glass policy according to standards set by Unilever and HACCP

• Demonstrated proper first-aid techniques to a class of 20-24 students• Developed excellent oral skills from teaching selected chapters from the Toronto EMS training manual • Construct accident scenarios in order to aid in the teaching of proper first aid techniques and assess student's ability to perform certain tasks

• Served as the main tutor for a small class of eight grade three students in the subject of mathematics• Prepared lesson plans in accordance to appropriate curriculum set out by TDSB• Supervised other tutors as they provided 1 on 1 tutoring for students• Ensured proper communication between parents, tutors, and students