Tamara Nicole Hube Escare Email & Phone Number

Wageningen, Gelderland, Netherlands

Wageningen, Gelderland, Netherlands

Ede, Gelderland, Netherlands

- Analysis of patient samples using the qRT-PCR technique for the detection of COVID-19- Analysis of food samples using Standard Operations Procedures

Wageningen, Güeldres, Países Bajos

Microbiome analysis from orchids from the rhizosphere and endosphere

Santiago, Región Metropolitana De Santiago, Chile

- Be in contact with laboratory equipment distribution companies to collect information about them, their quotes, and delivery times- Development of reports and keep the information organized in spreadsheets

Lo Aguirre

- Replacement during Q Fever emergency period- Obtaining nucleic acids from different organisms that can endanger national agronomy and agriculture for subsequent analysis either by conventional PCR, real-time PCR (Real Time 7300, 7500 program) and / or sequencing (BigDye Terminator, 3500 Series Genetic Analyzer)- Make a report of results and send it to the corresponding units- Preparation of inventory of both equipment and reagents and in quality control such as pipette calibration (Mettler Toledo) and equipment review

República, Santaigo, Chile

- Quotation, purchase and, organization of laboratory materials and reagents.- Order and inventory of material stored at ambient T °, -20 ° C, and -80 ° C.- Maintenance, order and, inventory of equipment, laboratory materials, and biological material.

República, Santiago, Chile

- Research work based on FONDECYT projects through the development of molecular biology techniques (PCR, EcoStudy real-time PCR, nucleic acid and protein electrophoresis, cloning), microbiology (Bacterial cultures in solid and liquid media, use of a lighter and flow hood laminar and stoves) and management of vegetable crops- Preparation of a corresponding analysis of the results to respond to the hypothesis raised- Guide intern students

República, Santiago Chile

Title: "Determination of the role of calreticulin 1 and 2 in the endoplasmic reticulum during the establishment of the immune response in Arabidopsis thaliana".Answer the hypothesis raised using techniques such as conventional PCR, semi-quantitative PCR (RT-PCR), real-time PCR (Ecostudy) and, Western Blots (Image Studio Digits ver. 4.0). For this, DNA and RNA extraction, cDNA synthesis, nucleic acid and, protein electrophoresis was carried out. In addition, infection tests (plant / pathogen) were performed. Statistical analyzes were carried out using GraphPad Prism and images were modified using Illustrator CC.

República

- Identification of mutant plants by genotyping- Management of plant material (Arabidospsis thaliana and Nicotianabemthamiana)- Gateway Cloning- Stable transformation of plants by floral dip

Santiago, Chile

- Preparation of umbilical cord blood samples for cryopreservation- Preparation of reports- Discussion of scientific articles