Brand lead for Oxemia (desidustat), a new chemical entity

Pharmaceutical Science Laboratory ( Neuropharmacology)• Studying the alternate JAK-STAT pathway elicited by binding of IL-6 and TNF-α to the nicotinic α7 receptor and their roles in the inflammatory signaling pathway• Research Project:- Study of chemical chaperones that increase the surface expression of nicotinic α7 receptor and the subsequent role of nicotinic α7 receptor in inflammation • Extensive experience with Tissue culture, Radioligand Binding Assays… Show more Pharmaceutical Science Laboratory ( Neuropharmacology)• Studying the alternate JAK-STAT pathway elicited by binding of IL-6 and TNF-α to the nicotinic α7 receptor and their roles in the inflammatory signaling pathway• Research Project:- Study of chemical chaperones that increase the surface expression of nicotinic α7 receptor and the subsequent role of nicotinic α7 receptor in inflammation • Extensive experience with Tissue culture, Radioligand Binding Assays α-BGT(Bungarotoxin) 125I - to check for the surface expression of a7 receptors, carrying out the periodic Iodination reactions and wipe tests • Gained significant experience with biochemical assays such as the SEAP Assay, BCA Assay, Luciferase and the MTS assay. Successfully carried out many Western blots to detect for the presence of a7 protein in varied cell lines• Have been designing my own protocols, trouble shooting experiments , statistically analyzing data and presenting the data every week. Show less

Pharmaceutical Science Laboratory (Behavioral pharmacology)• In vivo study of the chronic and toxic effects with respect to behavioral expression of tolerance to the drugs.• Screening the analgesic activity of Cannabinoid agonists in mice (Tail-Flick Method) and studying the effect of the same as diuretic agents in mice and rats

Tufts Medical School, Boston, Department of Pathology. Research Assistant• Concurrently worked on two projects:- 1) The ATG5 project- Studying the role of inflammatory mediators which are involved in SLE (Systemic Lupus Erythromatous) in TLR7 transgenic mice which are known to develop this disease. Trying to correlate the role of autophagy in SLE by using ATG5 K.O mice• 2) The HERV Project:- Delineating the role of HERV-K18,… Show more Tufts Medical School, Boston, Department of Pathology. Research Assistant• Concurrently worked on two projects:- 1) The ATG5 project- Studying the role of inflammatory mediators which are involved in SLE (Systemic Lupus Erythromatous) in TLR7 transgenic mice which are known to develop this disease. Trying to correlate the role of autophagy in SLE by using ATG5 K.O mice• 2) The HERV Project:- Delineating the role of HERV-K18, an endogeneous human retrovirus, in tumorigeneisis by working with HERV-K18 transgenic mice. Trying to understand the ways in which it influences human disease• Some of the Lab duties/skills involved- weekly genotyping of mice, excellent book keeping of genotyping and tissue culture records, isolating and maintaining primary murine tumor cell lines from spleen, thymus and peritoneum • Extensively carried out PCR for ATG5 and HERV-K18 and qPCR for TLR7 followed by Gel Electrophoresis to detect the amplified gene expression• Performed ELISAs and Antibody Nuclear Assays to detect for the presence of specific cell markers of HERV in TLR7 transgenic mice , also assisted in FAC(Flow-cytometry) analysis for sorting out the murine blood cells • Gained a lot of proficiency in tissue culture, DNA extraction and purification, PCRs and q-PCRs Show less

• Assisted in preparing medical literature for finished products.

• Co-monitored and wrote weekly reports of the ongoing clinical trials at various research centers and hospitals.• Reviewing the CRFs, IRB protocol , inventory control at the trial sites.• Good Knowledge of GCP/ICH guideliness.

Successfully initiated and coordinated 'The Yuva Jyot' movement, comprising of team of 200 student volunteers and helped out more than 350 blind students across the city in writing their annual examinations