My role in this project spins around using recombinant cells to produce some proteins and to purify them by affinity chromatography -AKTA purifier- to obtain different forms of that protein we think it has a novel role in the DNA repair pathway. Then to validate the DNA binding ability of this protein using electrophoretic mobility shift assay (EMSA) with DNA oligonucleotides I have radiolabeled by CTP, [α-32P] and ATP, [γ-32P]. We also aim to validate that in cancer cells to discover its… Show more My role in this project spins around using recombinant cells to produce some proteins and to purify them by affinity chromatography -AKTA purifier- to obtain different forms of that protein we think it has a novel role in the DNA repair pathway. Then to validate the DNA binding ability of this protein using electrophoretic mobility shift assay (EMSA) with DNA oligonucleotides I have radiolabeled by CTP, [α-32P] and ATP, [γ-32P]. We also aim to validate that in cancer cells to discover its biological role in resistance. I have participated with my team in the validation of some of our hypotheses, which will be published soon. Show less

My research proposal was about using miRNAs expression profiles as prognostic and/or diagnostic markers for liver cancer patients. I have worked on this project at the team of Prof. Dr. Abdelhady Abdelwahab the head of the Cancer biology department, National cancer institute of Egypt. As a research engineer. I had developed my practical skills in Molecular and cellular biology as nucleic acids extraction, 2D cell culture, MTT, RT-qPCR, and western blot.