Tarran Rupall Email & Phone Number

United Kingdom

Hinxton, England, United Kingdom

Hinxton, England, United Kingdom

Working within the Immune Genomics, Trynka group to investigate the genetic basis of autoimmunity in systemic lupus erythematosus (SLE). This is achieved by utilising techniques such as, cell culturing, flow cytometry, single cell RNA-sequencing and cell surface mapping.

Brighton, England, United Kingdom

Worked within the Newbury laboratory to investigate the interplay between stability and translation of lncRNAs. Used Drosophila as a model organism to identify lncRNAs that are differentially expressed and translated in RNA-seq and Poly-Ribo-seq data from exoribonuclease mutants. Identified novel short ORFs translated from lncRNAs and validated if their short ORFs are translated to discover the function of new small peptides.

Brighton, England, United Kingdom

Worked within the Lagnado laboratory investigating homeostatic synaptic plasticity at hippocampal synapses via imaging glutamate release. Transfected hippocampal cultures with EGFP-tagged glutamate receptors to detect glutamate release at synapses. Elicited chronic perturbations at synapses to monitor and investigate how homeostatic synaptic plasticity affects glutamate release. Confocal microscopy and Igor Pro, allowed me to detect an almost 2.5-fold decrease in the mean amplitude of glutamate released at synapses after treating hippocampal cultures with gabazine for 2 days.

Stevenage, England, United Kingdom

Worked within the Cell Line Development (CLD) department and gained industry experience in molecular and cellular biology, genetics, automated processes and data analysis. Optimised antibody production and cell line viabilities by performing sterile manual and automated cell culturing methods, which also prevented cell culture contaminations. Analysed antibody titer and CHO cell viabilities to monitor and assess multiple clones with the aim of identifying lead cell lines so project progression was maintained. Conducted experiments to assess the effects of different transfection methods in varying culture conditions on CHO cell viabilities and antibody titer production. This data allowed development of potential protocols to expedite workflow and potentially reduce project timelines. Performed molecular cloning and transfections of GSK monoclonal antibody plasmid constructs into CHO hosts to identify lead cell lines for manufacture. Assisted colleagues in single cell sorting and telomere-FISH experiments to maximise work efficiency and help keep to industry timelines.

Genetic Manipulation And Molecular Cell Biology (Msc), Molecular Genetics, Distinction

Neuroscience (Bsc), Neuroscience, First Class (1St)