Taylor Ralph Email & Phone Number

Raleigh, Nc, Us

Research Triangle Park

Developed and optimized bioassay and bioanalytical methods to enable sample testing and reporting. Leveraged prior cGMP/GLP experiences to help draft assay qualification plans and write group documents to build regulated lab environment. Designed ELN templates and infrastructure to support group assay work and reagent management (Benchling). Performed biochemical and cell-based assays to answer scientific questions for gene therapy programs. ➲ Written, reviewed, provided feedback, and assisted authors in the creation of quality documents to meet regulatory needs.➲ Designed ELN templates and trained users on ELN functions from assay work to reagent and equipment management.➲ Coordinated with IT on ELN development to suit group needs and facilitate onboarding of alternative ELN systems.➲ Developed and performed assays, such as enzymatic activity, potency, and total protein assays.➲ Optimized described test methods to a state suitable for in-house method transfer.➲ Acted as SME and point of contact for mentioned test methods post-transfer.➲ Worked within teams to meet project goals and deadlines for tasks ranging from sample analysis to assay development.➲ Demonstrated ability for both in-group and cross-functional knowledge transfer and independent work.

Morrisville, North Carolina, United States

Performed a variety of biological assays to support multiple project types, inclusive of purity testing and gene therapy-focused assays. ➲ Developed and validated methods to cGMP/GLP standards and provided critical feedback guiding project direction.➲ Executed assays to cGMP and GLP standards.➲ Performed PCR, ELISA, Cell Culture, and other bio-assays to support ongoing projects.➲ Developed and Validated testing methods to meet quality expectations. ➲ Worked within teams to meet project goals and deadlines.➲ Successfully and independently performed tasks as assigned.➲ Upheld and supported Good Documentation Practices for clear, concise, and transparent development of projects.

Chapel Hill, North Carolina, United States

Developed plasmids and produced AAV vectors to be used in the treatment of Hemophilia Types A and B. Managed and grew transgenic mouse colonies in support of ongoing experiments.➲ Developed customized DNA plasmids for use in AAV vectors to affect therapeutic change among Hemophiliac mice.➲ Produced AAV serotypes packaged with selected plasmids to introduce targeted genetic change in Hemophilia mouse models.➲ Performed cell-based assays to assess neutralizing antibody activity in blood sera. ➲ Exhibited proficiency in cell culturing of HEK 293 and Huh7 cell lines for use in cell-based assays and virus production.➲ Handled transgenic mice regularly for in-house husbandry and colony management.➲ Edited documents, proposals, and journal submissions for senior lab personnel.

Raleigh-Durham, North Carolina Area

Cultivated and observed different ecotypes of A. thaliana to identify an ecotype that contains a natural resistance to Geminiviridae, which plague Africa with potent crop diseases. ➲ Seeded, grew, and managed the health of the ecotypes leading up to and after their inoculation, notating the physical symptoms they suffered.➲ Conducted a simple DNA test using PCR and gel electrophoresis to confirm the cause of death was from the geminivirus that they were inoculated with.➲ Performed experiments on 40+ model plants species to identify natural resistances to crop diseases. Observational experiments were conducted by monitoring inoculated specimens for visible signs of resistance. ➲ Exhibited proficiency with the utilization of spectrophotometers and PCR devices. Executed assays such as PCR and Western Blot in support of ongoing projects in the lab.➲ Utilized peptide aptamers to investigate the conferral of disease resistance.➲ Analyzed biochemical changes undergone by target plant proteins.