● Conduct 3D bioprinting experiments with human iPSC-derived vascular and retinal progenitor cells to model the outer retinal blood barrier (oBRB) and age-related macular degeneration.● Design and implement an organ-on-a-chip system to analyze the microfluidic transport ability and permeability of retinal pigmented epithelial cells co-cultured with vascular endothelial cells.● Research the impact of polarized M1 and M2 macrophages on the development of ocular degeneration using a 3D… Show more ● Conduct 3D bioprinting experiments with human iPSC-derived vascular and retinal progenitor cells to model the outer retinal blood barrier (oBRB) and age-related macular degeneration.● Design and implement an organ-on-a-chip system to analyze the microfluidic transport ability and permeability of retinal pigmented epithelial cells co-cultured with vascular endothelial cells.● Research the impact of polarized M1 and M2 macrophages on the development of ocular degeneration using a 3D bioprinted oBRB model.● Assess the involvement of complement factor H (CFH) and the activated alternate pathway in the pathogenesis of AMD through the utilization of a 3D bioprinted oBRB model engineered with iPSCs. Show less

● Led comparative analysis of the differentiation potential between naïve converted ground state iPSCs and prime state iPSCs.● Developed a xeno-free, feeder-free system for the cultivation of clinically relevant iPSCs and progenitors.● Examined the regenerative capabilities of vascular and retinal progenitor cells derived from diabetic-induced pluripotent stem cells (iPSCs) in addressing diabetic vascular complications, including diabetic retinopathy.

● Produced nonviral, non-integrative induced pluripotent stem cells from human blood and fibroblasts.● Created a differentiation protocol to generate vascular progenitor cells from both human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) for the purpose of regenerating ischemic ocular diseases.● Cultivated hematopoietic progenitor cells using an in vitro hemogenic endothelium system derived from human pluripotent stem cells.

● Utilized porcine hepatocyte spheroids in the hollow fiber bioreactor for various applications.● Engaged in tissue engineering by working on hirudin and VEGF gene-transfected endothelial cells along with primary heart valve cells.

● Conducted metabolic assessments of tissue-engineered articular cartilage under different culture and seeding conditions.● Implemented auto and allografts of mesenchymal stem cell-polyglycolic acid (PGA) complex to address defects in canine and rabbit knees.