Bioinformatics focused on addressing systems-level control of glycosylation. Analysis of transcriptomic data to find altered glycosylation pathways and molecular subtypes of cancer. Bioinformatics development for high-throughput glycome profiling.

molecular cloning techniques to create plasmids for expression in bacterial systems. PCR reactions for amplifying DNA for recombination purposes. Used Restriction enzymes to digest DNA for analysis or use for plasmid construction. Used both Electroporation and Heat Shock methods for plasmid transformation into cellsuse of lambda red recombination machinery for genetic knockouts in E.colihelped in the design and implementation of CRISPR Cas9 guide RNAs for genetic… Show more molecular cloning techniques to create plasmids for expression in bacterial systems. PCR reactions for amplifying DNA for recombination purposes. Used Restriction enzymes to digest DNA for analysis or use for plasmid construction. Used both Electroporation and Heat Shock methods for plasmid transformation into cellsuse of lambda red recombination machinery for genetic knockouts in E.colihelped in the design and implementation of CRISPR Cas9 guide RNAs for genetic recombination applications in Clostridia and E.coli. use of Basic Local Alignment Search Tool (BLAST) to compare DNA sequences between mutant strains and wild type strains of bacteriapreparation of growth media for cells, inoculation of cells and monitoring of cell growth. Preparation of growth plates.Gel Electrophoresis for analysis of isolated DNA fragmentsDNA Isolation, purification, and quantification for downstream applications, such as recombination or sequencing. Show less