Gene therapy development, testing, and analysisMolecular assay developmentMammalian cell culture

• Development lead for novel rAAV-mediated gene-editing therapy for rare disease• Third generation (NGS) long-read sequencing (PacBio, Nanopore) methods development • Single molecule/single cell whole genome sequencing development• RNA isoform discovery (Iso-Seq, Capture-seq), transcriptomic and targeted RNA-seq development• Research and development into increasing AAV-mediated gene integration

• Dissertation Focus: Characterizing and predicting gene editing outcomes of mutagenic DNA double strand break repair at nucleotide-specific resolution• Specialization: High throughput CRISPR screens (in vivo and in vitro) and Next Gen Sequencing (NGS) methods development, large dataset analysis with R and python, mechanistic insights into DNA repair biology• Interests: cancer biology, NGS, gene editing methods development, DNA repair, nucleic acids secondary structures,

• Teaching assistant for multiple lecture and laboratory-based biology courses at the undergraduate level. • Experience: Molecular Biology Lecture, Experiments in Molecular Biology Lab, Genetics, and Introductory Biology. • Responsibilities: lecturing, mentoring, grading assignments, and preparing labs.

• Mentor and manager of five undergraduates in independent research projects and honors theses in the lab

• Project Manager for MassCONNECT.DH - a digital health startup incubator• Liaison between entrepreneurs, biotech mentors, investors, and MassBio. I also organize meetings and take minutes.• Working with Celata Bioinovations - AI drug discovery platform investigating post translationally modified proteins

• Sequencing Chemistry Team for the Qiagen GeneReader prior to commercial launch• Successfully supported operations for clinical compliance and verification• Sent to Qiagen headquarters in Hilden, Germany multiple times for Feasibility Campaign • Generated superior performance metrics for the GeneReader• Surpassed all thresholds for commercial release in half of the estimated time

• Authored institutional EHS protocols• Trained employees on environmental, health, and safety regulations and emergency action• Chair of the Safety Committee• Certified OSHA Blood-borne pathogens and hazardous waste

• Performed complex molecular genetics assays in Clinical Laboratory Improvement Amendment (CLIA) certified laboratory on highly sensitive human samples• Responsibilities included design, development, and validation of clinical assays for detection of genetic mutations• Sanger and next gen gene sequencing, PCR, gel electrophoresis, nucleic acids extraction, and fragment analysis

• Developed and authored institutional EHS protocols •Trained employees on EHS and emergency action procedures in a CLIA certified molecular genetics laboratory

• Performed manual and fully automated nucleic acids extractions • Performed all actions according to GLP and institutional protocols in a CLIA certified clinical laboratory for detection of rare genetic mutations in cancer patients and for prenatal testing

• Intern at the Harvard School of Dental Medicine/ Brigham and Women’s Hospital with Dr. Nathaniel Treister• Completed original research in Oral chronic Graft Vs. Host Disease (published September 2009)• Saw patients, assessed disease, performed data entry and analysis• Worked with pathology to investigate human samples