Undergraduate Research Associate
San Diego, Ca, Us
Single-Molecule Polymer Biophysics - Rae Anderson LabThe goal of this project was to study the complex stress-strain relationships in solutions of entangled actin filaments using optical tweezers. As the principal researcher on this project, I was tasked to build this project from the ground up; on my first day, I walked into a vacant lab containing only a desk and an optical table.To build a force-measuring optical tweezers fluorescence microscope, I:· Learned to program and use NI LabView and NI Data Acquisition to acquire data and control piezoelectric stage, piezoelectric mirror, position sensing photodiode, and CMOS camera.· Assembled optical tweezers by aligning an IR laser parallel and concentrically through custom built beam expanders, collimators, periscopes, and attenuators on two planes.· Learned to script in Matlab to automate analog signal analysis by outlier exclusion, sinusoidal regression fitting, differentiation, and noise reduction to resolve piconewton forces and calibrate optical tweezers. After building optical tweezers, I:· Designed, optimized, and verified custom biochemical protocols to polymerize fluorescent F-actin, conjugate fluorescent F-actin to fluorescent microspheres, and coat microspheres with streptavidin.· Designed, conducted, and analyzed experiments probing the effects of oscillation amplitude and frequency, microsphere diameter, and actin concentration on resistive force