Souvik Basak Email & Phone Number

Munich, By, De

Munich, Bavaria, Germany

Munich, Bavaria, Germany

• Conduct transcardial perfusion to extract and prepare brain samples• Brain slicing using a vibratome• Analyze neuronal projections through microscopy, quantifying fluorescence intensity using ImageJ• Statistically analyze data across multiple brain samples • Visualizing findings through graphical representation• Brain extractions from embryos and prepare samples using vibratome• Immunostaining on brain slices followed by imaging using fluorescence microscopy.

Munich, Bavaria, Germany

Munich, Bavaria, Germany

• Overwatching mice brain surgery procedures, including the insertion of EEG and EMG electrodes for the study of sleep and anesthesia effects on brain activity.• Engaging and conducting mice water maze experiments.• Assembling EEG and EMG electrodes onto PCB sockets.

Munich, Bavaria, Germany

Chennai, Tamil Nadu, India

PROJECT TITLE: Production and characterization of Polyhydroxybutyrate (PHB) from bacteria isolated from marshy soil.Grade: O• Isolated bacteria using serial dilution method and screened for PHB synthesizing bacteria using Sudan Black smear microscopy method.• PHB production and extraction, followed by the identification of the most potent isolate.Studied the effects of alternative carbon and nitrogen sources on PHB yield • Using Response surface methodology (RSM)… Show more PROJECT TITLE: Production and characterization of Polyhydroxybutyrate (PHB) from bacteria isolated from marshy soil.Grade: O• Isolated bacteria using serial dilution method and screened for PHB synthesizing bacteria using Sudan Black smear microscopy method.• PHB production and extraction, followed by the identification of the most potent isolate.Studied the effects of alternative carbon and nitrogen sources on PHB yield • Using Response surface methodology (RSM), designed a Central Composite Design (CCD) model for optimization of carbon and nitrogen sources, and pH for maximum PHB yield• Characterized the PHB by FTIR• Published a research paper — “PHB Production by Bacillus megaterium LSRB 0103 Using Cornstarch and Urea”• Published a review paper — “Metabolic flux analysis and metabolic engineering for polyhydroxybutyrate (PHB) production” Show less

Chennai, Tamil Nadu, India

Worked under the supervision of Prof. Dr. Lilly M Saleena.Completed 2 projects during this time.

Chennai, Tamil Nadu, India

PROJECT TITLE: Isolation, screening, and identification of Urease-producing bacteria from quarry soil sample.Grade: A+• Qualitatively screened soil samples for UPBs (Urease Producing Bacterias) using Christensens Urea Agar Base• Isolated 4 (UR01, UR02, UR03, UR04) strains from soil sample (collected from an agricultural field) • Quantified and evaluated the urease production by assaying the samples• Performed Gram staining, followed by DNA isolation, 16s rRNA gene… Show more PROJECT TITLE: Isolation, screening, and identification of Urease-producing bacteria from quarry soil sample.Grade: A+• Qualitatively screened soil samples for UPBs (Urease Producing Bacterias) using Christensens Urea Agar Base• Isolated 4 (UR01, UR02, UR03, UR04) strains from soil sample (collected from an agricultural field) • Quantified and evaluated the urease production by assaying the samples• Performed Gram staining, followed by DNA isolation, 16s rRNA gene amplification and sequencing, and identification of the isolates. Show less

Tambaram, Tamil Nadu, India

I learned about Biotechnology Industrial Equipments and how to operate them. Learned preparations of various types of broth and media LB broth/media, nutrient broth/media, etcPerformed sterilization of various media and pieces of equipment. Also conducted experiments to study the Bacterial Growth by inoculating fresh E.coli culture into LB media and using a benchtop incubator checked the Optical density (O.D.) of the sample using a Visible Spectophotomer at 560nm. Also studied the various… Show more I learned about Biotechnology Industrial Equipments and how to operate them. Learned preparations of various types of broth and media LB broth/media, nutrient broth/media, etcPerformed sterilization of various media and pieces of equipment. Also conducted experiments to study the Bacterial Growth by inoculating fresh E.coli culture into LB media and using a benchtop incubator checked the Optical density (O.D.) of the sample using a Visible Spectophotomer at 560nm. Also studied the various phases of a bacterial life cycle such as lag, log, stationary, and decline phases and plotted a growth curve indicating the same. Show less

Tambaram, Tamil Nadu, India

PROJECT TITLE: Screening of antibacterial activity, antioxidant activity, and phytochemical analysis of acetone leaf extract of 𝘖𝘳𝘵𝘩𝘰𝘴𝘪𝘱𝘩𝘰𝘯 𝘴𝘵𝘢𝘮𝘪𝘯𝘦𝘶𝘴• Obtained 50% Acetone extract of the plant Orthosiphon stamineus Soxhlet extractor• Evaluated the antibacterial activity of the sample by Well Diffusion Method, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) against a group of pathogens (3 bacterial strains – E. coli, B. subtilis… Show more PROJECT TITLE: Screening of antibacterial activity, antioxidant activity, and phytochemical analysis of acetone leaf extract of 𝘖𝘳𝘵𝘩𝘰𝘴𝘪𝘱𝘩𝘰𝘯 𝘴𝘵𝘢𝘮𝘪𝘯𝘦𝘶𝘴• Obtained 50% Acetone extract of the plant Orthosiphon stamineus Soxhlet extractor• Evaluated the antibacterial activity of the sample by Well Diffusion Method, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) against a group of pathogens (3 bacterial strains – E. coli, B. subtilis, S. aureus).• Determined the free radical scavenging capacity of DPPH against the sample using DPPH assay. Show less

Kolkata, West Bengal, India

My day to day role was writing blogs on various nutritional and medical/medicinal topics. I learnt a lot about various drugs, their uses and dosages, and also about various medical treatment possible to cure numerous disabilities.

Master Of Science - Msc., Molecular And Cellular Biology, 1.57

Bachelor Of Technology - Btech, Biotechnology, 9.12 Cgpa

Activities and Societies: Best Volunteerism Award (Batch 2019-23) Resource Management head-RARIORA’22 Resource Management head-RARIORA’23.

High School Diploma, Pcmb, 80.2% (Puc)

Activities and Societies: Science Quiz Runners up

93.4%

Activities and Societies: Vice Secretary of Hobby Club, Science Club, State level spell bee contestant