Thibaut Pollina Email & Phone Number

Morlaix, Fr

Morlaix, Brittany, France

Morlaix, Bretagne, France

The PlanktoScope is a modular, open-source hardware and software platform that allows for high-throughput quantitative imaging of plankton samples in aquatic biology and ecology. Its small size, ease of use, and low cost make it suitable for deployment in a range of applications, including the monitoring of laboratory cultures or natural micro-plankton communities. It can be controlled from any WiFi-enabled device, and its versatility allows for rapid reconfiguration to match the evolving needs of the user.Visit our website at : www.planktoscope.org

Stanford, United States Of America

In my role at Stanford as coordinator of the PlanktoScope project at the PrakashLab, I was responsible for inventing the first versions of the microscope and working with PrakashLab and Plankton Planet members to improve it. I had the opportunity to travel to various countries to test the microscope in different conditions. After publishing a paper on our findings, Adam Larson and I open-sourced the project, which led to the formation of a community of users that we supported in replicating the microscope and using it in their own projects.Supervisor : Manu Prakash

Pacific Ocean

As an oceanographic engineer on board the schooner Tara, I was responsible for ensuring the proper functioning and maintenance of the oceanographic instruments and sampling systems on board the ship. My job was to make sure that the in-line instruments, the optical instruments, the plankton nets, etc., are in perfect working order. I also had to ensure their maintenance, pre-process, pre-analyze and save the recorded data. I also tested the first prototype of the PlanktoScope in onboard condition.Supervisors : Guillaume Bourdin and Fabien Lombard

Station Biologique De Roscoff - Prakash Lab, Stanford University

In this position at Plankton Planet, I was responsible for imagining, designing and prototyping a microscope to be used by citizens on board a sailboat and capable of producing scientific quantitative imaging data. My work consisted in determining the technical specifications and functionalities of this microscope, as well as in designing and implementing it as a prototype. The goal of this project was to enable citizens to better understand and observe microscopic organisms in marine waters, and thus help protect and preserve these ecosystems.

Station Biologique De Roscoff

During this position, I implemented a morpho-genetic technical approach to image and isolate consortia involving cyanobacteria. Phycoerythrin, a pigment present in most cyanobacteria that forms an efficient fluorescent marker, was used to detect them. Using a microscope equipped with a micromanipulator, we were able to image and collect single eukaryotic cells showing association with cyanobacteria. We sorted more than 150 of them, present in the micro-plankton (size fraction 20-180 µm) of different oligotrophic water bodies. The 18S and 16S rDNA barcodes were sequenced from these holobionts to identify partners in each consortium. Using the global Tara Oceans data set, we will quantify the prevalence of these associations and analyze the correlation with biological and environmental parameters.Supervisors : Colomban de Vargas and Sebastien Colin

Station Biologique De Roscoff

During this internship, I implemented a morpho-genetic technical approach to image and isolate consortia involving cyanobacteria. Phycoerythrin, a pigment present in most cyanobacteria that forms an efficient fluorescent marker, was used to detect them. Using a microscope equipped with a micromanipulator, we were able to image and collect single eukaryotic cells showing association with cyanobacteria. We sorted more than 150 of them, present in the micro-plankton (size fraction 20-180 µm) of different oligotrophic water bodies. The 18S and 16S rDNA barcodes were sequenced from these holobionts to identify partners in each consortium. Using the global Tara Oceans data set, we will quantify the prevalence of these associations and analyze the correlation with biological and environmental parameters.Supervisors : Colomban de Vargas and Sebastien Colin

Région De Oslo, Norvège

I conducted this internship using transmission and scanning electron microscopy to visualize the cells. I prepared the samples by fixing and dehydrating them, then embedded them in resin and made ultra-thin sections. I visualized the sections with a transmission electron microscope. For scanning electron microscopy, I fixed the cells on filters and mounted them on stubs. I sputter-coated the samples with platinum and visualized them with a scanning electron microscope. This work will have been used in this article.Supervisor : Wenche Eikrem

Station Biologique De Roscoff

For this study, transcriptomes from 15 strains of Clade VII and 26 transcriptomes belonging to other clades or classes of green algae (Chlorophyta) were selected. These transcriptomes were produced in the framework of two projects: "Marine Microbiology Initiative" in the United States and "Oceanomics" in France. Sequencing was performed on Illumina Hi-Seq 2000, either as a single read of 100 nt or a double read of 2 x 50 nt. Raw data were assembled using Trinity and analyzed using several bioinformatics tools, including FastQC, riboPicker, RSEM, and Blastn. We used these data to measure transcriptome contamination and construct a phylogenetic tree of the selected strains. This work will have been used in this article.Supervisor : Daniel Vaulot

Station Méditerranéenne De L'Environnement Littoral De Sète

During my internship, I built a photosynthetron to measure oxygen on phytoplankton samples from 12 mesocosms subjected to different abiotic conditions. I also performed experimental monitoring by measuring chlorophyll and correlating the results to phytoplankton taxonomy. Finally, I measured the net oxygen production of the samples to assess the efficiency of photosynthesis.Supervisor : Francesca Vidussi

Master - Biologie Santé Ecologie, Environnement Et Gestion De La Biodiversité

Activities and Societies: Représentant des étudiantsThis course concerns terrestrial and aquatic environments, their biodiversity.

Licence, Hydrogéologie

Activities and Societies: Président de l'association "Sempervirentes"This training aims to provide students with the necessary skills.

Licence, Biologie Ecologie

Activities and Societies: Escalade / Randonnée / Energie Citoyenne / Vélo en TêteThis training covers biology, geosciences, chemistry.