Thomas Brefort Email and Phone Number

# Design and establish processes and technology platforms for scalable individualized mRNA cancer immunotherapy production, clinical trial biomarker analyses and R&D projects # Guide and drive the development and validation of innovative, scalable solutions for the individualized mRNA cancer vaccine process # Lead and develop the Genomics Research and Development teams

Aug 2023 - Oct 2023 Post Contractual Non-Compete Agreement#Lead Genomics’ NGS business in Europe, full responsibility for profit & loss, strategy, technologies, assets and staff at all sites# Orchestrate, lead, and develop cross-functional, multi-national matrix teams: Operations, Project Management, R&D, Bioinformatics, IT, Quality, Business Development, Sales# Guide and prioritize R&D and Bioinformatics teams in scouting,testing, and validation of novel technologies and automated sample preparation and analyses workflows#Acquire and manage pharma and biotech projects, clinical studies and trials# Build, implement and continuously optimize automated LIMS-controlled sample processing pipelines# Accomplished fast and profitable integration and restructuring of acquired companies and teams, realising 40% savings# 2019: Integrated GATC Biotech GmbH, LifeCodexx NIPT and Eurofins Genomics Europe NGS business into one LEAN, automated high throughput NGS competence center# Propel operational excellence and LEAN initiatives, continuously optimizing efficiency, productivity, and service offerings while maintaining highest quality

# Novel non-invasive molecular Biomarkers from body fluids, Discovery, Development and Validation: Circulating RNA, DNA, Protein, Liquid Biopsies# Molecular Biomarker Contract Research, Consulting and Solutions for Pharma, Customers # Translational Research, Molecular Diagnostics, Companion Diagnostics, Treatment Monitoring# Cardiovascular Diseases, Inflammatory/Autoimmune Diseases, Oncology/Cancer# Technology Scouting and Validation, Process Development and Optimization # Implementation of Quality Management (QC/QA)# ISO 17025 Accreditation# High throughput multiplex Methods and Platforms: - microRNA-, mRNA-, SNP-Profiling - Standardized, robust Preparation and Quantitation of microRNA/RNA from dilute body fluids - Microarrays, PCR, highthroughput quantitative realtime PCR, qRT-PCR, different multiplex multi-bead and multi-particle Assay, multiplexing, barcoding in targeted enrichment, clinical next generation sequencing (NGS)

as aforementioned # Additional Responsibility for Processes and Customer Consultation, Projects in Genomic Services# S1 Project Leader - Genetically Modified Organisms # Restructuring of Company to Comprehensive Biomarker Center

# Technology, Platform and Assay Application Development# Product Development# Development of High throughput multiplex Platform and Assays: - Microfluidic Microarray Platform Development: Automation, Programing, Instrument control, Biologic Assay Design, Development and Integration - miRNA, mRNA, SNP profiling, targeted enrichment for next generation sequencing (NGS), Quality Control Assays Implementation - multi-disciplinary Team (Software, Electronics, Engineering, Chemistry, Biology, MedTech) - PCR, qRT-PCR, NGS

# Fundamental Research in Pathogenomics, Phytopathology, Secretomics, Cell Signalling, Gene Regulatory Networks ...# Projecting, Responsibility for Postdocs, PhD, Diploma, Master and Bachelor students, Technical assistants and apprentices# Organization and teaching of University Lectures, Courses # National and International Cooperations# Presentations on numerous national and international Conferences # In depth Knowledge and hands-on Experience in: -Genomics: Genome Assembly, Genome Annotation, Genome Comparisons, BLAST etc., Microarrays, Trancriptomics, virtual Secretome, Protein Function Predictions, Proteomics - Molecular Biology: Molecular Cloning, Transformation (Bacteria, Fungi, Plants), Genetic Engineering, cDNA libraries, DNA-/RNA-/Protein Isolation, Northern, Southern Blotting, PCR, qRT-PCR, Yeast- 2-Hybrid, Design and Performance of high throughput Genetic Screening, DNA-Protein- Interactions, Electrophoretic Mobility Shift Assays, Sanger-Sequencing - Biochemistry: (heterologous) Protein Expression, Protein Affinity Purification, Chromatography, Protein Characterization, Immunoprecipitation, Western Blotting, Phosphorylation/Kinase Assays, Enzyme Kinetics - Cell Biology: Development and Implementation of cell-based assays (chromogenic, colorimetric, flurimetric), Plant Infections, Light Microscopy, Confocal Laser Scanning Microscopy