Thomas Glaze Email & Phone Number

Philadelphia, Pa, Us

Manage the material input (chemicals and other supplies) and output (finished products, e.g. kits, enzymes) of the biochemistry department. Perform and supervise the preparation of product components such as buffers and of finished products such as kits, enzymes, and antibodies. Develop SOPs that enable consistent reproducible performance and production of components and finished products. Maintain accurate records (date, quantity, assay results) of finished items prepared and of source materials (e.g. bulk enzyme stocks). Perform and supervise quality control assays using spectrophotometer and fluorescence microplate readers. Communicate restocking needs to Protein Production staff and may assist and collaborate in some preparations, assays, and quality control. Take initiative regarding product quality control analysis, troubleshooting, and the physical organization of the lab. Collaborate with team members to keep track of supplies and equipment maintenance needs and generate purchase orders. Create and maintain inventory of products using company-wide software Navision. Perform purification procedures and quantitative analysis on proteins and antibodies.

Responsible for maintaining, overseeing the laboratory, and involve in ordering supplies.Generated experimental data and databases.Utilized sterilization techniques.Set up and independently carry out biological and biochemical experiments:Dissected mice - harvested organs infected with Mycobacterium avium.Created DNA agarose plugs of Escherichia coli and M. avium and used pulse field gel electrophoresis (PFGE).Determined antibiotic resistance of E. coli in nursing home patients.Determined antibiotic resistance of Acinetobacter species in hospital patients.Basic tissue culture and PCR techniques.Minimum inhibitory concentration (MIC) studies of antibiotic resistance in E. coli and Acinetobacter.Vitek machine and API strips to determine the biochemical nature of test bacterium.