Thomas Redden Email & Phone Number

Natick, Massachusetts, United States

Utilized HPLC analysis to quantify analyte concentrations in client samples. Prepared samples for analysis through extraction. Prepared standard calibration samples to verify instrument data integrity. Documented all activity with a proprietary LIMS. Wrote automation scripts to interact LIMS data with HPLC software. Communicated with laboratory directors routinely to verify smooth operation.

Researched efficient mutagenesis of novel transcription factor MT-Bell4 in M. Truncatula. Performed analysis of ATAC sequencing data to create efficient Cas9 gRNA sites for mutagenesis, validated by in vivo knockout. Knockout procedure included creating plasmid construct through digestion & Golden Gate ligation, bacterial & plant culture and transformation. Programmed an automated liquid handler for high throughput genotyping and plasmid construction. Developed a custom pipeline template tool in Python that can be used to replicate NGS data analysis in parallel from public databases.

Instructed senior undergraduates to perform mutagenesis of plant tissue in Medicago Truncatula. Taught students how to perform PCR, vector ligation, bacterial & plant transformation, genotyping of plant tissue. Managed inventory and reagents needed.

Aided in the creation of a device that detects jet fuel contamination. Utilized UV-Vis spectrometry to capture readings of contaminants in jet fuel. Collaborated with engineers to build a device that could be utilized in the field to detect contamination at low concentration.

Created computer algorithms that analyze polymer movement in transmission electron microscope videos. Written in Java utilizing the Processing library, the program segments each part of the polymer body into nodes, and tracks how far they move between frames of the video.

Master Of Science - Ms, Cell/Cellular And Molecular Biology

Bachelor Of Science - Bs, Biomedical/Medical Engineering

Bachelor Of Science - Bs, Biochemistry And Molecular Biology