• Implementation of an upstream platform for mAbs production at various scales (15 mL, 10 L, 50 L, 200 L in single-use bioreactors).• Technology transfer of external production bioprocesses (gap analysis, scalability studies, equipment adjustment).• Technical support of the bioproduction unit (technology transfer, troubleshooting, training).• Involvement in collaborative projects (industrial expertise, planification, data generation and reporting, trainee management).• Launching of a development unit (equipment purchase, qualification, validation, SOP).• Lab management (orders, stock supply, newcomers training).

Viral vector production process scale down and mini-bioreactors platform establishmentAchievements: • Lentivirus and Adeno Associated Virus production in bioreactor (3 L, 10 L);• Implementation of a DASgip platform (250 mL bioreactor)Methods:Reusable bioreactor management (mounting, assembly lines, CIP), suspension cell culture, transfection, protocol and batch record writing, design of experiments, process data analysis.

Generation of knockout cell lines using CRISPR/Cas9 to study the role of RUNX transcription factors in prostate cancer.Achievements:• CRISPR/Cas9 guides design and production in bacteria (E.coli)• RUNX KO cell lines generation (HEK 293T, hPC-3, mES)• Molecular and cellular characterizationMethods:Cloning, DNA extraction, cell culture, transfection, flow cytometry, genotyping PCR, sanger sequencing, western-blot, confocal microscopy.Other:- Oral presentations and report writing in English

Discovering pharmaceutical industry and preparing orders in a warehouse