Construct, optimize and execute protocols to discover, identify, and select high titer monoclonal antibodies for our clients based upon research goals.Screen and affinity rank lead clones that are then sequenced for lead candidates.IACUC committee member maintaining onsite vivarium operations.

Project manage in vivo immunization campaigns utilizing the proprietary Alloy Gx mouse strain for the discovery and generation of novel human antibodies that support client therapeutic research programs.Assess the success of these immunization strategies via serum ELISA tests and cell binding via flow cytometry.Develop hybridomas cells that produce targeted antibody by fusing primary B cells from lymph nodes and/or splenocytes harvested from immunized mice, with immortal myeloma cells in cell culture.Screen supernatants for positive antibody selection via ELISA and/or flow cytometry analysis to identify positive hybridoma producing specified target monoclonal antibodies.Harvest all antibody positive hybridoma supernatants and hybridoma colonies for genetic sequencing before becoming a final deliverable product to clients.Keep all records of data and procedures in ELN and lab management storage systems.Manage and train co-ops and junior scientists with proper aseptic laboratory techniques and procedures followed by data collection and analysis.

Transduce HEK cells with packaged gamma retrovirus in order to produce novel virus stocks that further transduce target DNA creating human CAR T cells with metabolic bolt on genes.Designed, developed and implemented flow cytometry analysis of intracellular cytokine production of T cells and other surface staining screening efforts.Performed other T cell proliferation and cytotoxicity assays in presence of cultured target cancer cell lines.Coordinated with outside vendor to analyze samples for the development of a monoclonal recombinant antibody to be used for selectivity assays of our target.Interpreted data into conclusive results in order to make decisions delivered in high level and small team presentation which moved high priority programs forward under tight timelines.Maintained meticulous raw data, ELN and study report records.

Initiated and developed in vivo immunopharmacology studies in murine syngeneic and xenograft tumor models finalizing with complete data analysis.Evaluate immunology readouts such as deep dive immunophenotyping of tumors, phagocytosis assays, cytokine profiling for biomarker identification in early clinical development.Developed and perfected in vitro murine and human macrophage cells for phagocytosis assays cultured with tumor target cell lines testing various new molecules from the protein science group.Communicated with colleagues and senior scientists of newly obtained data to coordinate next experiments.Maintain data and records of experiments in ELN and data storage files.

Executed various assays, including flow cytometry and sorting, cell-based andimmune function/response assays and reporter assays to support in vitro and in vivo immuno-onclogy model development.Developed procedures to enhance sensitivity of ELISA assays detecting challenging targets from tumor lysates.Maintained detailed records of experimental protocols and data in laboratory notebooks inaccordance with company policy.

Starting position with company as a Research Associate until promotion in January 2013. Strong hands-on animal handling skills with laboratory animals particularly mice and rats (various routes of injections, restraining techniques, anesthesia methods, various small surgeries, animal monitoring, and sample collections). Dedicated to improve/develop in vivo oncology models such as breast, pancreatic, colorectal and some ovarian cancer, and auto immune disease models such as ITP, CAIA, CIA, and TGFNA induced diseases to determine therapeutic efficacy and potential mechanisms of action for drug candidates across portfolio.Proficient with Xenogen Spectrum in vivo imaging system applied to internal programs.Performed ex vivo analysis when needed with ELISA and/or flow cytometry analysis.Plan, design, coordinate, and execute experiments followed by complete data analysis, and written documentation of results in database and reports.Led in vivo ITP disease model and in vitro MSD assays to progress high priority program Nipocalimab (M281) for FDA IND submission.Collaborate with research associates and scientists across a range of disciplines and projects to achieve company goals under very tight and stringent timelines.

Perform studies on a daily basis for pharmokinetic research of cancer/infection drug treatments.Work with senior scientists and chemists to format experiments efficiently.Administer test articles via oral gavage, IV, IP, subcutaneous, and infusion for mice and rats.Collect specimen samples at determined time points to be analyzed.Prepare cultures and have aided in some necropsy procedures at a study's end.Prepare all vehicle dosing compounds needed for final dosages.Overtime and weekend support for various tumor model studies.

Lead technician of research studies supervising successful completion and accurate data collection.Administer test articles via oral gavage, IV, subcutaneous, or IM per study protocol.Performed observations for data collection of potential toxic effects of test article.Maintain animal care and report adverse health findings to veterinary staff.Collect blood and organ specimens through various methods and deliver to appropriate on site lab.Ensured quality of data of myself and other team members.

Responsible for safe operation of RTS and Gillig buses while a student at the University of Massachusetts, Amherst.Trained new drivers and held third highest training seniority upon departure.Supervised entire fleet of buses out on route while tending to problems and issues with buses, drivers, road condition, accidents, and relayed information to the publicKept data entries and official records up to date

Assisted in biodiversity monitoring of wildlife in the Amazon at Lago Preto, PeruIdientified, tracked and cataloged species in the area.Entered data into spreadsheets provided by supervising biologist.Research conducted to promote and further strengthen conservation of the area.Worked jointly with members of WCS-DICE.

Began as a volunteer nurse summer 2002 and hired officially the following September as my high school job.Assisted doctors and technicians in surgery, emergencies, take and process X-rays, assist in general appointments.Monitored boarding animals, post-surgery and critical care patients.Responsible for all boarding animals duties included feeding, cage cleaning, bathing, exercising, administering necessary medication, (oral, IV, and under the skin), and report all behavior to supervising veterinariansPrepared and analyzed lab cultures leading to diagnosis of patient.Filled out properly prescribed medications as per order of the attending veterinarians.Maintained overall sterility of entire hospital.