Proteomic Characterization of Blood Samples in Intensive Care Medicine

Analysis of the Secretome of Hepatic Stellate Cells in Response to Bacterial SepsisAs part of my master's thesis I analyzed the secretome of human hepatic stellate cells (HSCs) in response to free fatty acid loading and the stimulation with LPS. - Methods: LC-MS (Orbitrap Exploris 480), Label-free quantification, 4-Azido-L-homoalanine (AHA)-based click chemistryPatients with metabolic dysfunction-associated steatotic liver disease (MASLD) face heightened sepsis risk and (HSCs) play an important role in both conditions, as they not only emit immune responses after stimulation with LPS but also store lipids and are responsible for the onset and progression of fibrosis, exacerbating the severity of MASLD.

1) Establishing genetic pan-melanoma liquid- biopsy biomarkers in melanoma using methylation-specific PCR (MSP).2) Mechanisms of KDM5B in melanoma heterogeneity and plasticity

The effect of increased oxidative stress on the development of heart failure with preserved ejection fraction.