Freelance consultant offering on-site or remote expert support on all aspects of preclinical in vivo research in rodents. I have extensive experience with advanced rodent surgery, freely moving tracer-infusion models like glucose clamp and metabolic flux assessment, indirect calorimetry/energy balance studies, glucose telemetry, and much more. Consultancy services include:- On-site surgical training of staff.- On-site surgical services for preparation of ready-to-use operated animals.- Training on specific experimental techniques, for example glucose clamp, freely moving infusion models, or glucose telemetry.- In vivo experimental design, model development, and integration of different techniques in one model, like combining indirect calorimetry with vascular infusion/sampling catheters, or synchronizing food intake measurements with continuous glucose monitoring.- Development of data analysis methods and novel metrics from high-resolution continuous physiological data.- Design of surgical and experimental facilities, including advice on instruments, equipment, and experimental systems.

Co-organizer and senior instructor on the annual Rodent Glucose Clamp Course. Students are trained both in surgical catheterization (carotid artery and jugular vein) and glucose clamp study design and execution.

Senior scientist with a primary role in creating and consolidating the Rodent Metabolic Phenotyping Platform (RMPP), which offers expert support for all rodent studies at CBMR. Main architect behind creation of cutting-edge in vivo research facility, including:- State-of-the-art rodent surgical facility.- Climate controlled procedure room and housing for thermoneutrality and cold-challenges.- Facility design and scientific oversight of the entire renovation process. - Identified and led acquisition of the most advanced metabolic phenotyping technologies and experimental systems. Trained to expert level on all systems. Major Achievements:- Co-authored 14 papers in top journals, including Cell, PNAS, JCI, and JBC.- Close collaborations on innovation and development with leading suppliers of in vivo systems and surgical equipment, ensuring unique competitive advantages for CBMR researchers.- Mentoring and training of 4 scientists and 4 technicians in surgical and experimental skills required for their individual specialist roles.Key experimental models established:- Sable Promethion indirect calorimetry with stable isotope and methane analyzers and respirometry treadmills.- Catheter-based infusion and blood sampling in mice during measurements of respirometry and stable isotope enrichment (Sable systems).- Non-invasive and sample-free method for identification of diabetic mice in the home cage using the Tecniplast DVC system. - UID Mouse Matrix system for monitoring of individual temperature and activity in socially housed mice.- Chronic IV infusions in freely moving mice for toxicology and pharmacology studies.Surgical models established in mice:- Denervation of brown adipose tissue to switch off BAT thermogenesis.- Catheterization of internal carotid artery for infusions into the brain.- Partial hepatectomy for studies on liver regeneration.- Liver ischemia-reperfusion injury.- Gastric catheterization for “oral” dosing in freely moving mice.

Senior in vivo metabolic phenotyping specialist and animal lab manager in the Molecular Metabolism Lab (PI: Jonas T. Treebak) responsible for development and refinement of new surgical models and experimental techniques.Major achievements:- Co-authored 8 papers in top journals, including Diabetologia, Journal of Physiology, and Molecular Metabolism.- Developed a data analysis pipeline for comprehensive characterization of ambulatory continuous glucose monitoring data from mice.- Founded the annual joint CBMR/RRSSC Rodent Glucose Clamp Course together with René Remie of the RRSSC.Key experimental models established:- DSI continuous glucose telemetry in mice.- Research Diets BioDAQ E3 continuous monitoring of food and water intake.- Integration of CGM and BioDAQ E3 systems for simultaneous monitoring of blood glucose and food intake.- In vivo metabolic flux assessment in freely moving mice.- Serum transfusions for organ cross-talk studies.Surgical models established in mice:- DSI glucose telemetry transmitter implantation- Bile duct catheterization.- Femoral artery and vein catheterization.

Resident in vivo metabolic phenotyping specialist in the Molecular Metabolism Lab (PI: Jonas T. Treebak). Continued honing of surgical skills, and refinement and optimization of experimental techniques, focusing on characterization and reduction of confounding factors such as procedure-related stress, surgical trauma, and peri-operative infections.Major achievements:- Authored and co-authored 9 papers in top journals, including Nature, Diabetes, and American Journal of Physiology.- Completed implementation of glucose clamp in unrestrained, freely-moving mice.- Implementation of aseptic surgery according to clinical standards led to practically complete elimination of post-operative complications.- Perfected the jugular vein + carotid artery dual-catheter surgical procedure to consistently achieve near-100% success rates.- Co-organized two international symposiums on the application of the freely moving glucose clamp technique in rodents.

Biochemist and mouse surgeon trainee in the Molecular Metabolism Lab (PI: Jonas T. Treebak). Main task was to acquire surgical skills and establish the glucose clamp technique in freely moving conscious mice. Successfully established the dual-catheter surgical model for simultaneous sampling and infusion in mice (carotid artery and jugular vein).