Thomas M. Email & Phone Number

Rockville, Md

Embryology• Embryo Biopsy for Pre-implantation Genetic Diagnosis/Screening (PGD/PGS).• Insemination of human oocytes by intra-cytoplasmic sperm injection (ICSI).• Testicular Biopsy PESA/TESA/TESE analysis.• In Vitro Fertilization of human oocytes.• Denuding of human oocytes for IVF.• Transfer of frozen and fresh/active cycle human embryos.• Assisted hatching of human embryos for both biopsy and transfer.• Cryo-preservation and thawing of human embryos and oocytes.• Assessment of human embryos at multiple stages of development.• Assist in the retrieval of human oocytes for IVF.• Processing and preparation of human sperm for IVF.• Cryo-preservation and storage of human sperm for IVF.• Preparation of lab reagents and culture dishes for human oocytes and embryos.• Management of IVF lab inventory logistics.• Regular interaction with patients to answer questions and provide reassurance and comfort.

Gaithersburg, Md

Virology• Coordinated and performed cell-based assays (Western Blotting, luciferase, etc).• Isolated unique viral strains from mixed populations of viruses. • Performed REA and RCA testing of viral vectors to ensure stability of constructs.• Determined viral titers by Fluorescent Forming Units (FFU) Assay.• Evaluated the use of columns as a cost effective alternative to viral purification.• Performed viral transfections in adherent and suspension cell cultures.• Utilized DNA digestion techniques to prepare plasmid DNA for transfection.• Designed, coordinated, and conducted in vitro experiments to evaluate vector productivity, growth characteristics, genetic stability, and to identify replication deficiencies.Cell Culture• Coordinated and maintained adherent and suspension mammalian cell cultures. • Scaled adherent cell cultures in plates/flasks.• Scaled suspension cell cultures to volumes of 125mL to 8 Liters.• Generated working cell banks of adherent and suspension cell lines. • Coordinated cell culture transfers and liquid nitrogen storage/retrievals.• Prepared and cryo-preserved cells in liquid nitrogen inventories. • Thawed cryo-preserved cell lines and cultured for use. • Managed the auxiliary cell culture lab and its cell culture stocks.• Adapted cells traditionally grown with serum containing media to serum free media.General• Shipment of hazardous biologics to external sites / testing facilities.• Generated and maintained database records and laboratory notebooks.• Maintained laboratory equipment and general laboratory upkeep.• Prepared media, buffers, and miscellaneous laboratory reagents.• Adapted and optimized assays and processes to fit team needs.• Developed and presented timelines for meeting deliverables on multiple project teams.• Wrote/modified standard operating procedures.• Assisted in the training of junior and senior employees.*See also Research Assistant: Temporary for additional responsibilities.

Gaithersburg, Md

Virology• Propagated viral vectors using complementing suspension and adherent cell cultures.• Purified viral vectors by isopycnic gradients and tangential flow filtration.• Prepared samples using DNA extraction techniques and evaluated by PCR.• Performed gel electrophoresis on PCR products using both agarose and flash gels.*Began as a contracted employee through On Assignment Lab Support.**Refer also to Research Associate, as roles and responsibilities carried over and were increased.

Norfolk, Virginia Area

• Conducted environmental monitoring of laboratories.• Performed quality checks and documentation of finished products.• Performed in-process auditing of biomedical devices.• Audited physical inventories of biomedical devices.

• Maintained batch cultures of Propionibacterium Jensenii and isolated produced proteins.• Prepared various laboratory reagents, sterilized lab equipment, and maintained lab upkeep.• Proofread documents, filed paperwork, and sent/received faxes.

• Managed residence hall desks monitoring traffic, providing assistance, and maintaining dormitory security.• Provided assistance to parents, students, and faculty via telephone, email, or in person to ensure satisfaction and proper guidance.• Performed administrative duties such as the issuance of keys, maintenance of key inventories, submission of maintenance requests, and general telephone and service duties.